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wako125-05061赖氨酰肽链内切酶LysylEndopeptidase
【产品详情】
赖氨酰肽链内切酶
品牌：WAKO和光
级别：质谱级
wako125-05061赖氨酰肽链内切酶LysylEndopeptidase
英文品名：LysylEndopeptidase?,MassSpectrometryGrade
货号：125-05061
规格：520g
应用：蛋白质组学。
用途：赖氨酰肽链内切酶，蛋白测序、质谱分析，多肽合成
wako125-05061赖氨酰肽链内切酶LysylEndopeptidase
【实验原理】
赖氨酰肽链内切酶是质谱分析前处理时zui常用的蛋白分解酶即赖氨酰肽链内切酶，赖氨酰肽链内切酶可以特异性切除赖氨酸基团C末端的多肽，赖氨酰肽链内切酶可用于蛋白测序分析和Lys-X化合物的酶合成。
若同时使用赖氨酰肽链内切酶和胰酶，可更好地切断赖氨酸基团的多肽，增加多肽的数量。赖氨酰肽链内切酶已按照使用习惯做成小包装，是方便使用赖氨酰肽链内切酶的冷冻干燥品。
【产品特点】
赖氨酰肽链内切酶LysylEndopeptidase125-05061wako
1）赖氨酰肽链内切酶用于蛋白质谱分析：高特异性、高蛋白质消化率；
2）赖氨酰肽链内切酶能增加肽段数量、提高裂解效率；
3）赖氨酰肽链内切酶独立包装，20g/一支
【结果】表明Lep酶解的错误裂解率zui低。
Tp酶解时加入Lep后，错误裂解率有所降低，同时，赖氨酰肽链内切酶可以鉴定出更多的多肽。

赖氨酰肽链内切酶LysylEndopeptidase125-05061wako
Lep+Tp赖氨酰肽链内切酶切后，可以在m/z=2000时得到吸收峰，而单独的Tp酶切在m/z=2000时没有吸收峰。该结果表明Lep可以提高测序覆盖度。
赖氨酰肽链内切酶LysylEndopeptidase125-05061wako

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012-08643AminoAcidsMixtureStandardSolution,TypeB1mL5A-

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016-08641AminoAcidsMixtureStandardSolution,TypeB5mL-

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