ScienCellsGeneQuery™HumanWntSignalingPathwayqPCRArrayKit(GQH-WNT)isdesignedtofacilitategeneexpressionprofilingof88keygenesinvolvedinWntsignalingtransduction. TheWntsignalingpathwayiswellknownforitsroleincarcinogenesisandembryonicdevelopment. ThiskitfocusesoncanonicalWntsignaling. Briefexamplesofhowincludedgenesmaybegroupedaccordingtopurposeareshownbelow:
- Ligands: allknownWNTs
- Receptors: FZD1-10,SMO,LRP5/6,RSPO1-4,LGR4-6
- Receptortransducers: DVL1,DVL2,DVL3,CSNK1E,MARK1
- Transcriptionfactors: CTNNB1,TCF7L1,TCF7L2,LEF1,PYGO1
- Regulatoryelements: NLK,NKD1,AES,SFRP1,LRP1
GeneQuery™qPCRarraykitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatrecognizesandefficientlyamplifiesaspecifictargetgenescDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg2+andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsofthetargetgene,unlessotherwisenoted;and(iii)onlyonegeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis.
GeneQuery™HumanWntSignalingPathwayqPCRArrayPlateLayout*(8controls inBoldandItalic)
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | AES | CSNK2A1 | DKKL1 | FZD2 | GSK3A | LRP1 | PORCN | RSPO4 | SMO | WNT16 | WNT6 | β-actin |
B | APC | CTNNB1 | DVL1 | FZD3 | GSK3B | LRP5 | PPP2R4 | RYK | TCF7L1 | WNT2 | WNT7A | GAPDH |
C | AXIN1 | CTNNBIP1 | DVL2 | FZD4 | HNF1A | LRP6 | PYGO1 | SFRP1 | TCF7L2 | WNT2B | WNT7B | LDHA |
D | AXIN2 | CUL1 | DVL3 | FZD5 | KREMEN1 | MARK1 | ROR1 | SFRP2 | TLE1 | WNT3 | WNT8A | NONO |
E | BCL9 | DKK1 | FBXW7 | FZD6 | LEF1 | MARK2 | ROR2 | SFRP4 | WNT1 | WNT3A | WNT8B | PPIH |
F | CDC37 | DKK2 | FRZB | FZD7 | LGR4 | MARK3 | RSPO1 | SKP1 | WNT10A | WNT4 | WNT9A | GDC |
G | CSNK1A1L | DKK3 | FZD1 | FZD8 | LGR5 | NKD1 | RSPO2 | SKP2 | WNT10B | WNT5A | WNT9B | PPC |
H | CSNK1E | DKK4 | FZD10 | FZD9 | LGR6 | NLK | RSPO3 | SMARCA4 | WNT11 | WNT5B | ZNRF3 | NTC |
*geneselectionmaybeupdatedbasedonnewresearchanddevelopment
| CatalogNo. | GK026 |
|---|---|
| CountryofManufacture | UnitedStates |
| ProductCode | GQH-WNT |
| Size/Quantity | One96-wellplate,one20ulreactionperwell |
| ProductUse | GQH-WNTisforresearchuseonly.Itisnotapprovedforhumanoranimaluseorforapplicationinclinicalorinvitrodiagnosticprocedures. |
| Storage | Storeat4°Cuponreceipt.Forlong-termstorage(>1year),storetheplateat-20°Cinamanualdefrostfreezer. |
| ShippingInfo | Ambienttemperature. |
| 星形胶质细胞 | |
| 人星形胶质细胞生物学定量PCR芯片试剂盒 | 96tests |
| 人星形胶质细胞转录因子定量PCR芯片试剂盒 | 96tests |
| 基底细胞 | |
| 人基底细胞癌定量PCR芯片试剂盒 | 96tests |
| 内皮细胞 | |
| 人血管生成定量PCR芯片试剂盒 | 96tests |
| 人内皮细胞表型异质性定量PCR芯片试剂盒 | 96tests |
| 人内皮细胞生物学定量PCR芯片试剂盒 | 96tests |
| 人内皮细胞分化定量PCR芯片试剂盒 | 96tests |
| 人剪切应力和机械传导定量PCR芯片试剂盒 | 96tests |
| 上皮细胞 | |
| 人上皮细胞生物学定量PCR芯片试剂盒 | 96tests |
| 成纤维细胞 | |
| 人肺纤维化定量PCR芯片试剂盒 | 96tests |
| 人成纤维生长因子信号通路定量PCR芯片试剂盒 | 96tests |
| ***肤伤口愈合定量PCR芯片试剂盒 | 96tests |
| 肝细胞系统 | |
| 人肝癌定量PCR芯片试剂盒 | 96tests |
| 人丙型肝炎定量PCR芯片试剂盒 | 96tests |
| 人乙型肝炎定量PCR芯片试剂盒 | 96tests |
| 人肝脂肪变性定量PCR芯片试剂盒 | 96tests |
| 人肝星状细胞生物学定量PCR芯片试剂盒 | 96tests |
| 人先天性免疫应答定量PCR芯片试剂盒 | 96tests |
| 人自适应免疫反应定量PCR芯片试剂盒 | 96tests |
| 造血细胞 | |
| 人B细胞受体信号通路定量PCR芯片试剂盒 | 96tests |
| 人巨噬细胞生物学定量PCR芯片试剂盒 | 96tests |
| 人吞噬作用定量PCR芯片试剂盒 | 96tests |
| 人T细胞T细胞受体信号通路定量PCR芯片试剂盒 | 96tests |
| 人小神经胶质细胞生物学定量PCR芯片试剂盒 | 96tests |
| 人小神经胶质Sensome定量PCR芯片试剂盒 | 96tests |
| 人脂肪生成定量PCR芯片试剂盒 | 96tests |
| 人转录误调节在恶性血液肿瘤中的定量PCR芯片试剂盒 | 96tests |
| 黑色素细胞 | |
| 人黑素细胞发育与色素沉着定量PCR芯片试剂盒 | 96tests |
| 人黑素细胞细胞生物学定量PCR芯片试剂盒 | 96tests |
| 人黑色素瘤定量PCR芯片试剂盒 | 96tests |
| 神经细胞系统 | |
| 人星形胶质细胞生物学定量PCR芯片试剂盒 | 96tests |
| 人神经发育与再生定量PCR芯片试剂盒 | 96tests |
| 人星形胶质细胞转录因子定量PCR芯片试剂盒 | 96tests |
| 人神经可塑性定量PCR芯片试剂盒 | 96tests |
| 人神经传导与膜转运定量PCR芯片试剂盒 | 96tests |
| 肺细胞系统 | |
| 人小细胞肺癌定量PCR芯片试剂盒 | 96tests |
| 人非小细胞肺癌定量PCR芯片试剂盒 | 96tests |
| 人肺纤维化定量PCR芯片试剂盒 | 96tests |
| 肾细胞系统 | |
| 人肾癌定量PCR芯片试剂盒 | 96tests |
| 星形细胞 | |
| 人肝星状细胞生物学定量PCR芯片试剂盒 | 96tests |
| 人胰腺星状细胞生物学定量PCR芯片试剂盒 | 96tests |
| 细胞生物学 | |
| 人多能干细胞定量PCR芯片试剂盒 | 96tests |
| 人感知DNA损伤定量PCR芯片试剂盒 | 96tests |
| 人细胞生长和分裂定量PCR芯片试剂盒 | 96tests |
| 人细胞周期蛋白和周期蛋白依赖性激酶定量PCR芯片试剂盒 | 96tests |
| 甲状腺细胞系统 | |
| 人甲状腺癌和疾病定量PCR芯片试剂盒 | 96tests |
| 通路相关 | |
| 人血管生成定量PCR芯片试剂盒 | 96tests |
| 人细胞凋亡定量PCR芯片试剂盒 | 96tests |
| 人HIF1信号应答定量PCR芯片试剂盒 | 96tests |
| 人细胞吞噬作用定量PCR芯片试剂盒 | 96tests |
| 人感知DNA损伤定量PCR芯片试剂盒 | 96tests |
| 人细胞生长和分裂定量PCR芯片试剂盒 | 96tests |
| 人细胞周期蛋白和周期蛋白依赖性激酶定量PCR芯片试剂盒 | 96tests |
| 人先天性免疫应答定量PCR芯片试剂盒 | 96tests |
| 人自适应免疫反应定量PCR芯片试剂盒 | 96tests |
| 人B细胞受体信号通路定量PCR芯片试剂盒 | 96tests |
| 人细胞周期定量PCR芯片试剂盒 | 96tests |
| 人内吞作用定量PCR芯片试剂盒 | 96tests |
| 人内皮细胞分化定量PCR芯片试剂盒 | 96tests |
| 人剪切应力和机械传导定量PCR芯片试剂盒 | 96tests |
| 人缺氧应答定量PCR芯片试剂盒 | 96tests |
| 人黑素细胞发育与色素沉着定量PCR芯片试剂盒 | 96tests |
| 人神经发育与再生定量PCR芯片试剂盒 | 96tests |
| 人神经可塑性定量PCR芯片试剂盒 | 96tests |
| 人神经传导与膜转运定量PCR芯片试剂盒 | 96tests |
| 人非经典Wnt信号通路定量PCR芯片试剂盒 | 96tests |
| 人Notch信号通路定量PCR芯片试剂盒 | 96tests |
| 人T细胞T细胞受体信号通路定量PCR芯片试剂盒 | 96tests |
| 人Tie2/Angpt信号定量PCR芯片试剂盒 | 96tests |
| 人转录误调节在恶性血液肿瘤中的定量PCR芯片试剂盒 | 96tests |
| 人转录误调节在实体肿瘤中的定量PCR芯片试剂盒 | 96tests |
| 人Wnt信号通路定量PCR芯片试剂盒 | 96tests |
| 人Wnt信号目标基因定量PCR芯片试剂盒 | 96tests |
