DifferentiateEScellsintocardiacmyocytes Day-1:PassEScellsatnormaldensityongelatinizedplatetofreethecultureofcontaminationfibroblastcells. ____________________ Day1:Trypsinizedthecellsasfornormalpassaginguntilthecoloniesliftoff.Trytokeepthelooselyconnectedclumpsofcellstogetherbygentlehandling.Thendirectlyplatethecells1:3intobacterialgradePetridishesinLIFfreeEScellmedium. Day3:Aspiratethemediumcarefully.Avoidsuckinguptoomanyoftheaggregates.Thenaddnewmedium. Day5:AspirateasinDay3andreplacethemedium. Day7:Platethecellsinto24weeltissueculturegradeplate Day9:Changehalfofthemediumandobservebeating Day11:Changehalfofthemediumandobservebeating