Pre-madelentiviralparticlesexpressingafusiontargetof(RFP-humanActinORF). Thesequenceverifiedcodonsequenceofhuman betaactin(NCBIaccessionID:NM_001101)wasfusionedatitsN-terminalwithRFPprotein,expressedunderaconsitutivesuCMVpromoter,containingapuromycin antibiotic Markerunderthe RSVpromoter.Itcanbeusedto visualizesub-cellularstructurewheretheactinwastargeted.
Actinsarehighlyconservedproteinsthatareinvolvedincellmotility,structure,andintegrity.Thisactinisamajorconstituentofthecontractileapparatusandoneofthetwononmusclecytoskeletalactins.
Pleaseseeproductmanualfordetails.
Amount:200ul/vial(1×108IFU/ml), providedinDMEMmediumwith10%FBSand60ug/mlPolybrene(10x).
Cat#:LVP446-R