AmplideX® PCR/CE FMR1 Reagents
AmplideX PCR/CE FMR1 Reagents* are market-leading research tools for the detection of CGG repeats in the fragile X mental retardation (FMR1) gene. These reagents provide a PCR-only approach based on Triplet Repeat Primed PCR (TP-PCR) design to reliably amplify and detect all alleles including Full Mutations.
Features & Benefits
AmplideX PCR/CE FMR1 Reagents* have created an easy-to-use, accessible, high performance method for laboratories to reliably analyze CGG repeats and detect interrupting AGG sequences in the FMR1 gene.
Reduced ComplexityEase-of-analysis of the FMR1 gene has been simplified through:
- Implementation of proprietary PCR solution for amplifying GC-rich regions
- Automation of result calling using AmplideX PCR/CE FMR1 Reporter*
Optimized WorkflowValuable operator hands-on time has been significantly reduced through:
- Direct injection of PCR products (no PCR clean up) in to Capillary Electrophoresis platforms
- Decreased need for Southern blot analysis (up to 50 fold)
- End-to-end solution for FMR1 analysis including all necessary reagents and software
Quality PerformancePerforming FMR1 Analysis with Greater Sensitivity and Accuracy:
- Detection of all allele expansions, including low abundance full mutation size mosaics with up to at least 1300 CGG repeats
- Up to 875 fold more sensitive than Southern blot1
- Resolution of female homozygous and heterozygous samples and indication of interrupting AGG sequences
- Proven performance as indicated by more than 30 peer reviewed publications
*For Research Use Only. Not for use in diagnostic procedures.
Product Description
Analytical Characteristics of AmplideX PCR/CE FMR1 Reagents*:
- Detects all alleles including low abundance full mutations (Figure 1)
- Accurately sizes any repeat up to 200 CGG repeats (Figure 2)
- Resolves female zygosity (Figure 3)
- Detects presence of AGG interruptions (Figure 4)
Figure 1: Amplification of Asuragen’s Methylation and Sensitivity Control which has a 5% full mutation in a background of 95% Normal
Figure 2. Female premutation sample with accurate sizing of Normal (30 CGG) and Pre mutation allele (56 CGG)
Figure 3: The difference in the “stutter” peak patterns of homozygous and heterozygous female provides a clear resolution of zygosity
Figure 4. Female Full Mutation sample with AGG interruptions as indicated by sudden decrease in peak heights of the “stutter” peak profile
Ordering
Product Name | Number of Reactions | Catalog Number |
---|---|---|
AmplideX PCR/CE FMR1 Control | 24 UL | 49513 |
AmplideX mPCR FMR1 Control | 24 UL | 49514 |
AmplideX PCR/CE FMR1 Reagents | 100 | 49402 |
AmplideX mPCR FMR1 Kit | 24 | 49442 |
AmplideX PCR/CE FMR1 Reporter | N/A | 49576 |
T 1-877-777-1874; 512-681-5200 F 512-681-5202 E orders@asuragen.com
References
- Referenced in over 30 peer reviewed publications and used in over 200 laboratories, the AmplideX® PCR/CE FMR1 Reagents* are globally recognized as best-in-class for assessment of CGG repeats in the FMR1 gene.
- Key resources
- Videos
AmplideX® PCR/CE FMR1 Kit
AmplideX PCR/CE FMR1 Kit is an in vitro diagnostic (IVD) device for use in clinical laboratories for detection of the CGG repeats in the fragile X mental retardation (FMR1) gene. The device is intended to aid in the diagnosis of fragile X syndrome and fragile X associated disorders, e.g. tremor and ataxia syndrome (FX-TAS) and primary ovarian insufficiency (FXPOI), through determination of CGG repeat length up to 200 CGG and detection of alleles greater than 200 CGG. The kit provides a PCR-only approach based on Triplet Repeat Primed PCR (TP-PCR) design to reliably amplify and detect all alleles including Full Mutations.
Features & Benefits
AmplideX PCR/CE FMR1 Kit has created an easy-to-use, accessible, high performance method for laboratories to reliably analyze CGG repeats and detect interrupting AGG sequences in the FMR1 gene.
Reduced ComplexityEase-of-analysis of the FMR1 gene has been simplified through:
- Implementation of proprietary PCR solution for amplifying GC-rich regions
- Automation of result calling using AmplideX PCR/CE FMR1 Reporter
Optimized WorkflowValuable operator hands-on time has been significantly reduced through:
- Direct injection of PCR products (no PCR clean up) in to Capillary Electrophoresis platforms
- Decreased need for Southern blot analysis (up to 50 fold)
- End-to-end solution for FMR1 analysis including all necessary reagents and software
Quality PerformancePerforming FMR1 Analysis with Greater Sensitivity and Accuracy:
- Detection of all allele expansions, including low abundance full mutation size mosaics with up to at least 1300 CGG repeats
- Up to 875 fold more sensitive than Southern blot1
- Resolution of female homozygous and heterozygous samples and indication of interrupting AGG sequences
- Proven performance as indicated by more than 30 peer reviewed publications
Product Description
Analytical Characteristics of AmplideX PCR/CE FMR1 Kit:
- Proven clinical accuracy compared to Southern Blot (Table 1)
- Detects all alleles including low abundance full mutations (Figure 1)
- Accurately sizes all alleles up to 200 CGG repeats (Figure 2)
- Resolves female zygosity (Figure 3)
- Detects presence of AGG interruptions (Figure 4)
Table 1: Diagnostic Sensitivity of 100%; Diagnostic Specificity of 98.4% and Overall Accuracy of 99%*These 2 samples presented premutation alleles by both methods and low intensity full mutation alleles detected only by the AmplideX PCR/CE FMR1 Kit
Figure 1: Amplification of Asuragen’s Methylation and Sensitivity Control which has a 5% full mutation in a background of 95% Normal
Figure 2. Female Pre-mutation sample with accurate sizing of Normal (30 CGG) and Pre-mutation allele (56 CGG)
Figure 3: The difference in the “stutter” peak patterns of homozygous and heterozygous female provides a clear resolution of zygosity
Figure 4. Female Full Mutation sample with AGG interruptions as indicated by sudden decrease in peak heights of the “stutter” peak profile
Ordering
Product Name | Number of Reactions | Catalog Number |
---|---|---|
AmplideX PCR/CE FMR1 Control* | 24 UL | 49513 |
AmplideX mPCR FMR1 Control* | 24 UL | 49514 |
AmplideX PCR/CE FMR1 Reagents* | 100 | 49402 |
AmplideX PCR/CE FMR1 Kit | 100 | 76008 |
AmplideX mPCR FMR1 Kit* | 24 | 49442 |
AmplideX PCR/CE FMR1 Reporter* | N/A | 49576 |
T 1-877-777-1874; 512-681-5200 F 1-512-681-5202 E orders@asuragen.com
References
- Referenced in over 30 peer reviewed publications and used in over 200 laboratories, the AmplideX® PCR/CE FMR1 Reagents* are globally recognized as best-in-class for assessment of CGG repeats in the FMR1 gene.
- Key resources
- Videos
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各位战友好,因实验要求需要提取人的唾液DNA,但因标本需要在临床采集,无法做到当天就提取DNA,因此天根等漱口水提DNA的试剂盒都不满足实验需求。
之前一直在用艾德莱的唾液DNA保存运输提取试剂盒,但是这段时间下来感觉提取效率太低,而且特别不稳定,经常50%以上的标本都提不出来DNA,或者浓度特别低,完全崩溃的感觉!所以想更换试剂盒或者直接提供唾液DNA的保存液。另国外的这种试剂盒太贵了,因经费问题无法承受,所以希望战友们能够推荐一家效率高些的试剂盒,小女子不胜感激!!
有试剂A和B,传说10-30分钟就可以提取出来。不知道属不属实啊?
而且1000次580块钱!如果有用过的,可否跟我传授一下心得啊?
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