Highlights
- Quick (20 minute), large-scale recovery of ultra-pure DNA from PCR, endonuclease digestions, cell-free lysates, etc.
- ZR-96 Silicon-A Plate design allows DNA to be eluted at high concentrations into minimal volumes of solvent.
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc.
Description
Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
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Elution Volume | ≥ 30 µl for shallow well, ≥ 10 µl for deep well |
Equipment | Centrifuge with microplate carriers |
Purity | A260/A280 > 1.8 |
Sample Source | DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. |
Size Range | 75 bp to 23 kb for shallow well, 50 bp to 23 kb for deep well |
Yield | ≤ 5 µg total DNA can be recovered.For DNA 75 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
Q1: What is the lower limit and minimal amount of DNA that can be recovered?
Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.
Q2: How to process naked DNA stored in DNA/RNA Shield?
Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.
Q3: What to do if ethanol addition to the DNA Wash Buffer was omitted?
The DNA will be eluted off the column. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.
Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.
Q5: How many times can columns be reloaded?
We recommend no more than 5 times as binding efficiency might decrease.
Q6: What is the minimum input volume of DNA sample?
Working with volumes below 50 µl can result in decreased recovery. We recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.
Cat # | Name | Size | Price | |
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D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 | |
D4004-1-L | DNA Binding Buffer | 100 ml | $57.00 | |
D4003-2-48 | DNA Wash Buffer (Concentrate) | 48 ml | $60.00 | |
C2001 | Silicon-A Plate | 2 Plates | $129.00 | |
C2003 | Elution Plate | 2 Plates | $19.00 | |
C2002 | Collection Plate | 2 Plates | $22.00 |
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1. 超净台:单人单面的,垂直层流。
2. 倒置显微镜:够基本的观察用就行(但效果要好),无需成像之类的。
3. 液氮罐:两个,30-50升左右的。
4. 用于玻璃移液管的电动移液器一支。
前三个东东都国产的就行,电动移液器可以是进口的,大家给推荐一下,哪个厂家的哪个型号的哪个性价比好些~~(money不多哦)
:):):)
不胜感激!!!!
祝大家研究顺利!!!
怀着欣喜激动的心情做实验,可是。。。。。可是。。。。。超净台紫外灯忘记关了。。。。。痛心疾首,感觉不会再爱了,我连冻存都没冻过。。。。。
请各位大神指点焦虑的菜鸟,我的细胞系被照了四十多分钟,会有多大的影响,能不能继续用?对人的皮肤会不会有影响,现在过去了几个小时皮肤还挺正常的。
感谢!
2、关闭紫外灯,打开风机;
3、消毒液消毒台面、手
4、无菌操作
5、整理台面,关闭风机
暂无品牌问答