Description
Product Highlights
- Fast – simple protocol for isolation of high quality total RNA, genomic DNA and proteins in as little as 60 minutes
- Efficient – RNA, DNA and protein can be recovered from samples precious or biopsy samples through sequential isolation
- Convenient – an easy-to-use green dye enables easy determination of the phases for improved pipetting accuracy
- Flexible – reagent volume can be scaled for effective extraction of small to large input amounts of cells and tissue
- Consistent – protocol eliminates lysate splitting, thereby improving repeatability of results and increasing yield
Product Description
TRIsure provides a simple, efficient method for the sequential isolation of total RNA, genomic DNA and proteins from a wide variety of starting materials.
By combining thiocyanate compounds with phenol, TRIsure facilitates disruption of cells during homogenization and effectively inhibits DNase and RNase activity. After homogenizing the sample with TRIsure, chloroform is added and the homogenate is allowed to separate into three phases: a clear aqueous phase (upper) containing the RNA, a green colored organic phase (lower) and an interphase, containing the DNA and proteins. The RNA is extracted from the aqueous phase by isopropyl alcohol precipitation, DNA is precipitated from the organic layer with ethanol and the proteins are precipitated from the phenol-ethanol supernatant by isopropyl alcohol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities and then resuspended for use in downstream applications.
TRIsure allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population.
The simplicity of the TRIsure method allows simultaneous processing of a large number of samples and can be used in a wide variety of downstream applications including expression profiling, epigenetics, genotyping, transgenic analysis and cell line characterization.
Applications
- qPCR and RT-qPCR
- End-point PCR and RT-PCR
- Northern/Southern/Western, dot and slot blotting
- Genotyping
- Gene expression analysis
- Transgenic analysis
- Epigenetics
- Cell line characterization
- 2D gel electrophoresis
- Mass spectroscopy
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
RO逆渗透是一种通过目前国际流行的反渗透等办法,对原水进行过滤处理(物理法)后不添加任何化合物而生产出可供人类直接饮用的纯净水机器(也称为终端净水设备)。
采用水质符合中国卫生部《生活饮用水水质卫生规范》(2001)规定的市政自来水为原水,通过2个活性炭滤芯(1个颗粒活性炭、1个烧结活性炭)1个PPF溶喷滤芯对原水进行预过滤,再对预过滤水施加压力令其通过孔径大小为万分之一微米的RO(反渗透,英文Reverse Osmosis)膜,最后通过材质为果壳(椰壳)的载银活性碳(又名小T33)调节水的酸碱度(使制出的纯净水口感变得甘甜醇美)而生产出纯净水。
净水器使用砂滤、活性炭、超滤等技术,能在一定程度上净化水源。能去除泥沙、铁锈,但无法彻底去除水垢、细菌、病毒,属于初级过滤。使用KDF技术的净水器,能去除细菌、病毒,部分去除重金属,但无法去除水垢。
软水机基本都是采用离子交换技术,用钠型阳离子置换水中的钙镁离子(水碱),彻底去除水垢,可以给生活带来很多好处。
纯水机采用反渗透膜过滤技术,核心是微孔过滤,只有水分子和比水分子小的矿物质能通过膜并保留下来供饮用;细菌、病毒、水垢、污染物均不能通过膜,成为浓缩水排出;从而达到纯净水的标准。
主要看你具体的需要,
适合你的才是最好的。
我想咨询的是ELIX的处理柱(好象叫PRO-GUARD),多长时间换一次,我们这里在用水不是很大量的情况下半年就不行了,总是提示低压报警,现在刚换了三个月,又开始报警了,是否我们这里水质极差还是机器有问题?
该怎么解决呢,一个柱子近3000大洋,太贵了,还不如直接买纯净水划的来
一、净水机:
1、功能:能够过滤掉自来水氯、杂质等,最后出来的水能够达到生饮的标准,保留了自来水中的对人体有用的矿物质;
2、特点:没有电机,不需要电源,靠水压驱动过滤。没有储水罐,比较好的为五级过滤,第一级为滤芯,第二和第三级为活性炭,第四级为中空丝膜或陶瓷过滤,第五级为精致活性炭,主要用于改善口感。
二、纯水机:
功能:过虑掉自来水中所有物质,出来的水是纯净水,相当于我们买的纯净水,跟净水机相比,净水机只能过虑到杂质之类,纯水机还把水中的各种矿物质都过虑掉了,比如水里面对人体有害的各种重金属,净水机出来的水虽然有矿物质,但是只是自来水含有的,很多矿物质是自来水无法提供的,也无法过滤掉各种矿物质,如果希望纯水机出来的水含有矿物质,可以单加一个麦饭石,这样出来的就是矿物质比较丰富的矿泉水了,当然,这样的矿泉水肯定比净水机出来的可以生饮的水好。
纯水机的价格影响因素除了进口品牌和国产品牌的差异外,主要取决于产量、用途以及其他特殊要求。
综合来看,除非有硬性规定或者需要采用进口品牌来提升实验室形象,否则建议选择国产品牌即可,因为实际上纯水机作为实验室常规设备,在工艺技术上知名度较高的国产品牌已足与进口相媲美,且价格仅为进口的1/3左右。
你需要先确定采购水机的主要实验用途,不同的实验用途,对水的要求是不一样的,从而也决定了所采购纯水机的配置,进而决定价格。
水中需纯化的物质主要有:无机离子、总有机碳(TOC)、微小粒子、微生物(内毒素、热原)、弱电解质。根据对以上物质的去除度,生产出来的纯水分为三个级别:初级纯水、中级水、超纯水。一般来水,仅产初级产水的纯水机价格在1.2万左右(产量10L);同时产初级纯水和超纯水的纯水机价格在(2-3万左右,产量10L)。
此外,还需要确定具体的实验用途,不同的实验用水,对于水中的物质去除度是有区分的,会有一定的倾斜度,比如细胞培养型实验,就对无机离子和TOC去除度要求严格;无机分析实验的话对无机离子去除要求高。
国产中,锐思捷、力康都还不错。
暂无品牌问答