商品信息
联系客服
郑重提醒:
无质量问题不接受退换货,下单前请仔细核对信息。
下单后请及时联系客服核对商品价格,订单生效后再付款。
Highpuritydyed,solubleAzo-CM-Celluloseforthemeasurementofenzymeactivity,forresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
Substrateforthespecificmeasurementofendo-1,4-β-D-glucanase(cellulase).
Newchromogenicsubstratesfortheassayofalpha-amylaseand(1→4)-β-D-glucanase.
McCleary,B.V.(1980).CarbohydrateResearch,86(1),97-104.
LinktoArticle
ReadAbstract
Newchromogenicsubstrateshavebeendevelopedforthequantitativeassayofalpha-amylaseand(1→4)-β-D-glucanase.Thesewerepreparedbychemicallymodifyingamyloseorcellulosebeforedyeing,toincreasesolubility.Afterdyeing,thesubstrateswereeithersolubleorcouldbereADIlydispersedtoformfine,gelatinoussUSPensions.Assaysbasedontheuseofthesesubstratesaresensitiveandhighlyspecificforeitheralpha-amylaseor(1→4)-β-D-glucanase.Themethodofpreparationcanalsobeappliedtoobtainsubstratesforotherendo-hydrolases.
Evaluationofsubstratecompositionforlignocellulolyticenzymesproductionbysolidstatefermentationfromwastesofoliveoilandwineindustries.
Salgado,J.M.,Moreira,C.,Abrunhosa,L.,Venâncio,A.,Domínguez,J.M.&Belo,I.(2012).AmericanprogrammeforScience,TechnologyandDevelopment,95-101.
LinktoArticle
ReadAbstract
Wastesfromoliveoilandwineindustries(asexhaustedgrapemark,vineshoottrimmings,two-phaseolivemillwaste,vinassesandolivemillwastewaterwereevaluatedforlignocellulolyticenzymesproduction(ascellulases,xylanasesandferuloylesterases)bysolidstatefermentationwithAspergillusniger,AspergillusibericusandAspergillusjaponicus.TostudytheeffectofdifferentsubstratesinenzymesproductionaPlackett-Burmanexperimentaldesignwaspresented.Thevariablesthathadahigherpositiveeffectinlignocellulolyticenzymeswereurea,timeandexhaustedgrapemark.Themixtureoftwo-phaseolivemillwastewithexhaustedgrapemarkandvineshoottrimmingshadmaximaactivityofcellulases,xylanasesandferuloylesterases.
CellulolyticpotentialofThermophilicspeciesfromfourfungalorders.
Busk,P.K.&Lange.L.(2013).AMBExpress,3(1),47.
LinktoArticle
ReadAbstract
ElucidationoffungalbiomassdegradationisimportantforunderstandingtheturnoverofBIOLOGicalmaterialsinnatureandhasimportantimplicationsforindustrialbiomassconversion.Inrecentyearstherehasbeenanincreasinginterestinelucidatingthebiologicalroleofthermophilicfungiandincharacterizationoftheirindustriallyusefulenzymes.Inthepresentstudyweinvestigatedthecellulolyticpotentialof16thermophilicfungifromthethreeascomyceteordersSordariales,EurotialesandOnygenalesandfromthezygomyceteorderMucoralesthuscoveringallfungalordersthatincludethermophiles.Thermophilicfungiaretheonlydescribedeukaryotesthatcangrowattemperaturesabove45°C.All16fungiwereabletogrowoncrystallinecellulosebuttheirsecretedenzymesshowedwidelydifferentcellulolyticactivities,pHoptimaandthermostABIlities.Interestingly,incontrasttopreviousreports,wefoundthatsomefungisuchasMelanocarpusalbomycesreadilygrewoncrystallinecelluloseandproducedcellulases.Theseresultsindicatethattherearelargedifferencesinthecellulolyticpotentialofdifferentisolatesofthesamespecies.Furthermore,alltheselectedspecieswereabletodegradecellulosebutthedifferencesincellulolyticpotentialandthermostabilityofthesecretomedidnotcorrelatetothetaxonomicposition.PCRamplificationandsequencingof22cellulasegenesfromthefungishowedthatthelevelofthermostabilityofthecellulose-degradingactivitycouldnotbeinferredfromthephylogeneticrelationshipofthecellulases.
TranscriptionalcomparisonofthefilamentousfungusNeurosporacrassagrowingonthreemajormonosaccharidesD-glucose,D-xyloseandandL-arabinose.
Li,J.,Lin,L.,Li.H.,Tian,C.&Ma,Y.(2014).BiotechnologyforBiofuels,7(1),31.
LinktoArticle
ReadAbstract
Background:D-glucose,D-xyloseandL-arabinosearethethreemajormonosaccharidesinplantcellwalls.Completeutilizationofallthreesugarsisstillabottleneckforsecond-generationcellulolyticbioethanolproduction,especiallyforL-arabinose.However,littleisknownaboutgeneexpressionprofilesduringL-arabinoseutilizationinfungiandacomparisonofthegenome-widefungalresponsetothesethreemajormonosaccharideshasnotyetbeenreported.Results:Usingnext-generationsequencingtechnology,wehaveanalyzedthetranscriptomeofN.crassagrownonL-arabinoseversusD-xylose,withD-glucoseasthereference.WefoundthatthegeneexpressionprofilesonL-arabinoseweredramaticallydifferentfromthoseonD-xylose.ItappearsthatL-arabinosecanrewirethefungalcellmetabolicpathwaywidelyandprovoketheexpressionofmanykindsofsugartransporters,hemicellulasegenesandtranscriptionfactors.Incontrast,manyfewergenes,mainlyrelatedtothepentosemetabolicpathway,wereupregulatedonD-xylose.TherewiredmetabolicresponsetoL-arabinosewassignificantlydifferentandwiderthanthatundernocarbonconditions,althoughthecarbonstarvationresponsewasinitiatedonL-arabinose.Threenovelsugartransporterswereidentifiedandcharacterizedfortheirsubstrateshere,includingoneglucosetransporterGLT-1(NCU01633)andtwonovelpentosetransporters,XAT-1(NCU01132),XYT-1(NCU05627).Onetranscriptionfactorassociatedwiththeregulationofhemicellulasegenes,HCR-1(NCU05064)wasalsocharacterizedinthepresentstudy.Conclusions:WeconductedthefirsttranscriptomeanalysisofNeurosporacrassagrownonL-arabinoseandperformedacomparativeanalysiswithcellsgrownonD-xyloseandD-glucose,whichdeepenstheunderstandingoftheutilizationofL-arabinoseandD-xyloseinfilamentousfungi.ThedatasetgeneratedbythisresearchwillbeusefulforminingtargetgenesforD-xyloseandL-arabinoseutilizationengineeringandthenovelsugartransportesidentifiedaregoodtargetsforpentoseuntilizationandbiofuelsproduction.Moreover,hemicellulaseproductionbyfungicouldbeimprovedbymodifyingthehemicellulaseregulatordiscoveredhere.
Relevanceofthelightsignalingmachineryforcellulaseexpressionintrichodermareesei(hypocreajecorina).
Gyalai-Korpos,M.,Nagy,G.,Mareczky,Z.,Schuster,A.,Réczey,K.&Schmoll,M.(2010).BMCResearchNotes,3,330.
LinktoArticle
ReadAbstract
Background:Innature,lightisoneofthemostimportantenvironmentalcuesthatfungiperceiveandinterpret.Itisknownnotonlytoinfluencegrowthandconidiation,butalsocellulasegeneexpression.WethereforestudiedtherelevanceofthemaincomponentsofthelightperceptionmachineryofTrichodermareesei(Hypocreajecorina),ENV1,BLR1andBLR2,forproductionofplantcellwalldegradingenzymesinfermentationsaimedatefficientbiosynthesisofenzymemixturesforbiofuelproduction.Findings:Ourresultsindicatethatdespitecultivationinmostlydarkconditions,allthreecomponentsshowaninfluenceoncellulaseexpression.Whilewefoundtheperformanceoftheenzymemixturesecretedbyadeletionmutantinenv1tobeenhanced,thehighercellulolyticactivityobservedforΔblr2ismainlyduetoanincreasedsecretioncapacityofthisstrain.Δblr1showedenhancedbiomassaccumulation,butduetoitsobviouslylowersecretioncapacitystillwastheleastefficientstraininthisstudy.Conclusions:Weconcludethatwithrespecttoregulationofplantcellwalldegradingenzymes,thebluelightregulatorproteinsareunlikelytoactasacomplex.Theirregulatoryinfluenceoncellulasebiosynthesisinvolvesanalterationofproteinsecretion,whichmaybeduetoadjustmentoftranscriptionorposttranscriptionalregulationofupstreamfactors.Incontrast,theregulatoryfunctionofENV1seemstoinvolveadjustmentofenzymeproportionstoenvironmentalconditions.
DehydrogenaseGRD1RepresentsaNovelComponentoftheCellulaseReguloninTrichodermareesei(Hypocreajecorina).
Schuster,A.,Kubicek,C.P.&Schmoll,M.(2011).AppliedandEnvironmentalMicrobiology,77(13),4553-4563.
LinktoArticle
ReadAbstract
Trichodermareesei(Hypocreajecorina)isnowadaysthemostimportantindustrialproducerofcellulaseandhemicellulaseenzymes,whichareusedforpretreatmentofcellulosicbiomassforbiofuelproduction.Inthisstudy,weintroduceanovelcomponent,GRD1(glucose-ribitoldehydrogenase1),whichshowsenzymaticactivityoncellobioseandpositivelyinfluencescellulasegenetranscription,expression,andextracellularendo-1,4-β-D-glucanaseactivity.grd1isdifferentiallytranscribedupongrowthoncelluloseandtheinductionofcellulasegeneexpressionbysophorose.Thetranscriptionofgrd1iscoregulatedwiththatofcel7a(cbh1)underinducingconditions.GRD1isfurtherinvolvedincarbonsourceutilizationonseveralcarbonsources,suchasthoseinvolvedinlactoseandD-galactosecatabolism,inseveralcasesinalight-dependentmanner.WeconcludethatGRD1representsanovelenhancerofcellulasegeneexpression,whichbycoregulationwiththemajorcellulasemayactviaoptimizationofinducingmechanisms.
Influenceofthecarbonsourceonproductionofcellulases,hemicellulasesandpectinasesbyTrichodermareeseiRutC-30.
Olsson,L.,Christensen,T.M.I.E.,Hansen,K.P.&Palmqvist,E.A.(2003).EnzymeandMicrobialTechnology,33(5),612-619.
LinktoArticle
ReadAbstract
ThegrowthandenzymeproductionbyTrichodermareeseiRutC-30usingdifferentlignocellulosicmaterialsascarbonsourcewereinvestigated.Cellulose,sugarbeetpulpandalkalineextractedsugarbeetpulp(resultinginpartialremovalofhemicellulose,ligninandpectin)ormixturesthereofwereusedascarbonsources.Itwasfoundthatendoglucanaseandendoxylanseactivitieswereproducedthroughoutthecultivations,whereasα-arabinosidasewasinducedlateduringthecultivation.Thehighestamountsofendoglucanse,couldbemeasuredwhenT.reeseiRutC-30wasgrownoncelluloseorcellulosecontainingmixtures.Endoxylanasewasproducedonallsubstrates,butthepresenceofcellulosewasfavourablefortheproduction.Polygalacturonaseactivitycouldbemeasuredathighvaryinglevelsthroughoutthecultivations,exceptduringgrowthoncellulose.Thevaryinglevelsmightoriginatefromtheproductionofdifferentisoenzymesofpolygalacturonase.
Jeongeupianaejangsanensisgen.nov.,sp.nov.,acellulose-degradingbacteriumisolatedfromforestsoilfromNaejangMountaininKorea.
Yoon,J.H.,Choi,J.H.,Kang,S.J.,Choi,N.S.,Lee,J.S.&Song,J.J.(2010).InternationalJournalofSystematicandEvolutionaryMicrobiology,60(3),615-619.
LinktoArticle
ReadAbstract
AGram-stain-negative,motile,rod-shaped,cellulose-degradingbacterialstrain,BIO-TAS4-2T,whichbelongstotheBetaproteobacteria,wasisolatedfromforestsoilfromNaejangMountain,Korea,anditstaxonomicpositionwasinvestigatedbyusingapolyphasicstudy.StrainBIO-TAS4-2TgrewoptimallyatpH7.0–8.0,at30°Candinthepresenceof0–1.0 %(w/v)NaCl.Phylogenetictreesbasedon16SrRNAgenesequencesshowedthatstrainBIO-TAS4-2TclusteredwithmembersofthegeneraAndreprevotia,SilvimonasandDeefgeaofthefamilyNeisseriaceae,withwhichitexhibited16SrRNAgenesequencesimilaritiesof93.5–94.2 %.StrainBIO-TAS4-2TcontainedQ-8asthepredominantubiquinoneandsummedfeature3(C16:1ϖ7cand/oriso-C15:02-OH)andC16:0asthemajorfattyacids.TheDNAG+Ccontentwas63.8mol%.StrainBIO-TAS4-2Tcouldbedifferentiatedfrommembersofphylogeneticallyrelatedgenerabydifferencesinfattyacidcomposition,DNAG+Ccontentandsomephenotypicproperties.Onthebasisofphenotypic,chemotaxonomicandphylogeneticdata,strainBIO-TAS4-2Tisconsideredtorepresentanovelspeciesinanewgenus,forwhichthenameJeongeupianaejangsanensisgen.nov.,sp.nov.isproposed,withBIO-TAS4-2T(=KCTC22633T=CCUG57610T)asthetypestrain.
Regulationofendo-actingglycosylhydrolasesinthehyperthermophilicbacteriumThermotogamaritimagrownonglucan-andmannan-basedpolysaccharides.
Chhabra,S.R.,Shockley,K.R.,Ward,D.E.&Kelly,R.M.(2002).AppliedandEnvironmentalMicrobiology,68(2),545-554.
LinktoArticle
ReadAbstract
ThegenomesequenceofthehyperthermophilicbacteriumThermotogamaritimaencodesanumberofglycosylhydrolases.Manyoftheseenzymeshavebeenshowninvitrotodegradespecificglycosidesthatpresumablyserveascarbonandenergysourcesfortheorganism.However,becauseofthebroadsubstratespecificityofmanyglycosylhydrolases,itisdifficulttodeterminethephysiologicalsubstratepreferencesforspecificenzymesfrombiochemicalinformation.Inthisstudy,T.maritimawasgrownonarangeofpolysaccharides,includingbarleyβ-glucan,carboxymethylcellulose,carobgalactomannan,konjacglucomannan,andpotatostarch.Inallcases,significantgrowthwasobserved,andcelldensitiesreached109cells/ml.Northernblotanalysesrevealeddifferentsubstrate-dependentexpressionpatternsforgenesencodingthevariousendo-actingβ-glycosidases;thesepatternsrangedfromstrongexpressiontonoexpressionundertheconditionstested.Forexample,cel74(TM0305),ageneencodingaputativeβ-specificendoglucananse,wasstronglyexpressedonallsubstratestested,includingstarch,whilenoevidenceofexpressionwasobservedonanysubstrateforlam16(TM0024),xyl10A(TM0061),xyl10B(TM0070),andcel12A(TM1524),whicharegenesthatencodealaminarinase,twoxylanases,andanendoglucanase,respectively.Thecel12B(TM1525)gene,whichencodesanendoglucanase,wasexpressedonlyoncarboxymethylcellulose.Anextracellularmannanaseencodedbyman5(TM1227)wasexpressedoncarobgalactomannanandkonjacglucomannanandtoalesserextentoncarboxymethylcellulose.Anunexpectedresultwasthefindingthatthecel5A(TM1751)andcel5B(TM1752)genes,whichencodeputativeintracellular,β-specificendoglucanases,wereinducedonlywhenT.maritimawasgrownonkonjacglucomannan.Toinvestigatethebiochemicalbasisofthisfinding,therecombinantformsofMan5(Mr,76,900)andCel5A(Mr,37,400)wereexpressedinEscherichiacoliandcharacterized.Man5,aT.maritimaextracellularenzyme,hadameltingtemperatureof99°Candanoptimuntemperatureof90°C,comparedto90and80°C,respectively,fortheintracellularenzymeCel5A.WhileMan5hydrolyzedbothgalactomannanandglucomannan,noactivitywasdetectedonglucansorxylans.Cel5A,however,notonlyhydrolyzedbarleyβ-glucan,carboxymethylcellulose,xyloglucan,andlicheninbutalsohadactivitycomparabletothatofMan5ongalactomannanandhigheractivitythanMan5onglucomannan.ThebiochemicalcharacteristicsofCel5A,thefactthatCel5AwasinducedonlywhenT.maritimawasgrownonglucomannan,andtheintracellularlocalizationofCel5Asuggestthatthephysiologicalroleofthisenzymeincludeshydrolysisofglucomannanoligosaccharidesthataretransportedfollowinginitialhydrolysisbyextracellularglycosidases,suchasMan5.
CellulosedegradationbySulfolobussolfataricusrequiresacell-anchoredendo-β-1-4-glucanase.
Girfoglio,M.,Rossi,M.&Cannio,R.(2012).JournalofBacteriology,194(18),5091–5100.
LinktoArticle
ReadAbstract
Asequenceencodingaputativeextracellularendoglucanase(sso1354)wasidentifiedinthecompletegenomesequenceofSulfolobussolfataricus.Theencodedproteinsharessignaturemotifswithmembersofglycosidehydrolasesfamily12.Afteranunsuccessfulfirstattemptatcloningthefull-lengthcodingsequencesinEscherichiacoli,anactivebutunstablerecombinantenzymelackinga27-residueN-terminalsequencewasgenerated.This27-amino-acidsequenceshowssignificantsimilaritywithcorrespondingregionsinthesugarbindingproteinsAraS,GlcS,andTreSofS.solfataricusthatareresponsIBLeforanchoringthemtotheplasmamembrane.Astrategybasedonaneffectivevector/hostgeneticsystemforSulfolobusandonexpressioncontrolbythepromoteroftheS.solfataricusgenewhichencodestheglucosebindingproteinallowedproductionoftheenzymeinsufficientquantitiesforstudy.Infact,theenzymeexpressedinS.solfataricuswasstableandhighlythermoresistantandshowedoptimalactivityatlowpHandhightemperature.Theproteinwasdetectedmainlyintheplasmamembranefraction,confirmingthestructuralsimilaritytothesugarbindingproteins.TheresultsoftheproteinexpressioninthetwodifferenthostsshowedthattheSSO1354enzymeisendowedwithanendo-β-1-4-glucanaseactivityandspecificallyhydrolyzescellulose.Moreover,italsoshowssignificantbutdistinguishablespecificitytowardseveralothersugarpolymers,suchaslichenan,xylan,debranchedarabinan,pachyman,andcurdlan.
Unravellingthemolecularbasisforlightmodulatedcellulasegeneexpression-theroleofphotoreceptorsinNeurosporacrassa.
Schmoll,M.,Tian,C.,Sun,J.,Tisch,D.&Glass,N.L.(2012).BMCgenomics,13(1),127.
LinktoArticle
ReadAbstract
Background:Lightrepresentsanimportantenvironmentalcue,whichexertsconsiderableinfluenceonthemetabolismoffungi.StudieswiththebiotechnologicalfungalworkhorseTrichodermareesei(Hypocreajecorina)haverevealedaninterconnectionbetweentranscriptionalregulationofcellulolyticenzymesandthelightresponse.Neurosporacrassahasbeenusedasamodelorganismtostudylightandcircadianrhythmbiology.WethereforeinvestigatedwhetherlightalsoregulatestranscriptionalregulationofcellulolyticenzymesinN.crassa.Results:WeshowthattheN.crassaphotoreceptorgeneswc-1,wc-2andvvdareinvolvedinregulationofcellulasegeneexpression,indicatingthatthisphenomenonisconservedamongfilamentousfungi.ThenegativeeffectofVVDonproductionofcellulolyticenzymesistherebyaccomplishedbyitsroleinphotoadaptationandhenceitsfunctioninWhitecollarcomplex(WCC)formation.Incontrast,theinductionofvvdexpressionbytheWCCdoesnotseemtobecrucialinthisprocess.Additionally,wefoundthatWC-1andWC-2notonlyactasacomplex,butalsohaveindividualfunctionsupongrowthoncellulose.Conclusions:Genomewidetranscriptomeanalysisofphotoreceptormutantsandevaluationofresultsbyanalysisofmutantstrainsidentifiedseveralcandidategeneslikelytoplayaroleinlightmodulatedcellulasegeneexpression.Geneswithfunctionsinaminoacidmetabolism,glycogenmetabolism,energysupplyandproteinfoldingareenrichedamonggeneswithdecreasedexpressionlevelsinthewc-1andwc-2mutants.Theabilitytoproperlyrespondtoaminoacidstarvation,i.e.up-regulationofthecrosspathwaycontrolproteincpc-1,wasfoundtobebeneficialforcellulasegeneexpression.OurresultsfurthersuggestacontributionofoxidativedepolymerizationofcellulosetoplantcellwalldegradationinN.crassa.
Characterisationofcellulaseactivityinthedigestivesystemoftheredclawcrayfish(Cheraxquadricarinatus).
Xue,X.M.,Anderson,A.J.,Richardson,N.A.,Anderson,A.J.,Xue,G.P.&Mather,P.B.(1999).Aquaculture,180(3),373-386.
LinktoArticle
ReadAbstract
Endogenouscellulaseactivitywasidentifiedinthegastricfluidanddigestiveglandoftheredclawcrayfish.CellulaseshowedmaximalactivityfrompH4to5andwasstableforupto2hat40°C.Cellulaseactivityinthedigestiveglandwasunaffectedbyantibiotictreatment.Takentogetherthesefindingssuggestasignificantendogenouscomponentforredclawcellulaseactivity.Partialpurificationofcellulaseactivitywasperformedusinganionexchangeandgelfiltrationchromatography.OnemajorandoneminorbandofactivitywereidentifiedsubsequentlybySDS-PAGEandzymography.Themolecularweightofthemajorbandwasestimatedat40kDawhiletheminorbandwasestimatedat30kDa.Redclawcellulaseenzymesdemonstratedbroadsubstratespecificity,hydrolysingpolysaccharidescontainingβ-1,4andmixedβ-1,4andβ-1,3glycosidicbondsbutshowedapreferenceforsolublesubstrates.Hydrolysisproductsofcellodextrinsofvariouslengthsalsoshowedthattheenzymesliberatedfreeglucose.Exposureofredclawtoantibioticsresultedinadramaticdeclineinbacterialpopulationsinthegastriccontents(>90%)butonlya40%declineincellulaseactivity.
Identificationofthermostableβ-xylosidaseactivitiesproducedbyAspergillusbrasiliensisandAspergillusniger.
Pedersen,M.,Lauritzen,H.K.,Frisvad,J.C.&Meyer,A.S.(2007).BiotechnologyLetters,29(5),743-748.
LinktoArticle
ReadAbstract
TwentyAspergillusstrainswereevaluatedforproductionofextracellularcellulolyticandxylanolyticactivities.Aspergillusbrasiliensis,A.nigerandA.japonicasproducedthehighestxylanaseactivitieswiththeA.brasiliensisandA.nigerstrainsproducingthermostableβ-xylosidases.Theβ-xylosidaseactivitiesoftheA.brasiliensisandA.nigerstrainshadsimilartemperatureandpHoptimaat75°CandpH5andretained62%and99%,respectively,oftheseactivitiesover1hat60°C.At75°C,thesevalueswere38and44%,respectively.WhereasA.nigerisawellknownenzymeproducer,thisisthefirstreportofxylanaseandthermostableβ-xylosidaseproductionfromthenewlyidentified,non-ochratoxin-producingspeciesA.brasiliensis.
TheeffectofPleurotusostreatusarabinofuranosidaseanditsevolvedvariantinlignocellulosicbiomassesconversion.
Marcolongo,L.,Ionata,E.,Cara,F.L.,Amore,A.,Giacobbe,S.,Pepe,O.&Faraco,V.(2014).FungalGeneticsandBiology,72,162-167.
LinktoArticle
ReadAbstract
ThefungalarabinofuranosidasefromPleurotusostreatusPoAbfrecombinantlyexpressedinPichiapastorisrPoAbfanditsevolvedvariantrPoAbfF435Y/Y446Fweretestedfortheireffectivenesstoenhancetheenzymaticsaccharificationofthreelignocellulosicbiomasses,namelyArundodonax,corncobsandbrewer’sspentgrains(BSG),afterchemicalorchemical–physicalpretreatment.AlltherawmaterialsweresubjectedtoanalkalinepretreatmentbysoakinginaqueousammoniasolutionwhilstthebiomassfromA.donaxwasalsopretreatedbysteamexplosion.Thecapabilityofthewild-typeandmutantrPoAbftoincreasethefermentablesugarsrecoverywasassessedbyusingtheseenzymesincombinationwithdifferent(hemi)cellulolyticactivities.TheseenzymaticmixtureswereeitherentirelyofcommercialoriginorcontainedthecellulasefromStreptomycessp.G12CelStreprecombinantlyexpressedinEscherichiacoliinsubstitutiontothecommercialcounterparts.TheadditionofthearabinofuranosidasesfromP.ostreatusimprovedthehydrolyticefficiencyofthecommercialenzymaticcocktailsonallthepretreatedbiomasses.ThebestresultswereobtainedusingtherPoAbfevolvedvariantandarerepresentedbyincreasesofthexyloserecoveryupto56.4%.Thesedataclearlyhighlighttheimportantroleoftheaccessoryhemicellulolyticactivitiestooptimizethexylanbioconversionyields.
GrowthandEnzymeProductioninBlueCrabs(Callinectessapidus)FedCelluloseandChitinSupplementedDiets.
Allman,A.L.,Williams,E.P.&Place,A.R.(2017).JournalofShellfishResearch,36(1),283-291.
LinktoArticle
ReadAbstract
Thebluecrab[Callinectessapidus(Rathbun,1896)]isabenthicdecapodwithavarieddiet.Thedietincludesinvertebratesanddetritalmaterialthatcanhaverelativelylargeamountsofchitinandcellulose,bothofwhichcanbedifficulttodigestformanyorganismsandoftenrequiretheaidofspecificbacteriainthegutmicrobiome.Inthisstudy,juvenilebluecrabswerefedanoptimizeddefinedpelleteddietwitha20%replacementofwheatflourfillerwitheitherchitin,cellulose,ora14%/6%mixofboth,followedbyadietswitchtotheopposingingredient.Crabshadincreasinggrowthperformancewithincreasingamountsofcelluloseinthedietversuschitinandhadanadditionalmoltinmostcases.Thisoccurredduringtheinitialphaseandfollowingtheswitch,indicatingthatperformancecanberecovered.Subsequently,celluloseandchitindigestionassayswereusedtoshowthattheforegut,midgut,andhindgutwereallabletosignificantlydigestmorecellulosethanchitinwiththemajorityofactivityintheforegutandmidgut.Implicationsforrearinganddietformulationsaswellastheroleofcelluloseandchitindigestioninthenaturaldietarediscussed.
Conferringcellulose-degradingabilitytoYarrowialipolyticatofacilitateaconsolidatedbioprocessingapproach.
Guo,Z.P.,Duquesne,S.,Bozonnet,S.,Cioci,G.,Nicaud,J.M.,Marty,A.&O’Donohue,M.J.(2017).BiotechnologyforBiofuels,10(1),132.
LinktoArticle
ReadAbstract
Background:Yarrowialipolytica,oneofthemostwidelystudied“nonconventional”oleaginousyeastspecies,isunabletogrowoncellulose.Recently,weidentifiedandoverexpressedtwoendogenousβ-glucosidasesinY.lipolytica,thusenablingthisyeasttousecello-oligosaccharidesasacarbonsourceforgrowth.Usingthisengineeredyeastplatform,wehavenowgonefurthertowardbuildingafullycellulolyticY.lipolyticaforuseinconsolidatedbioprocessingofcellulose.Results:Initially,differentessentialenzymecomponentsofacellulasecocktail(i.e,.cellobiohydrolasesandendoglucanases)wereindividuallyexpressedinY.lipolyticainordertoascertaintheviabilityofthestrategy.Accordingly,theTrichodermareeseiendoglucanaseI(TrEGI)andII(TrEGII)weresecretedasactiveproteinsin Y.lipolytica,withthesecretionyieldofEGIIbeingtwicethatofEGI.CharacterizationofthepurifiedHis-taggedrecombinantEGproteins(rhTrEGs)revealedthatrhTrEGIdisplayedhigherspecificactivitythanrhTrEGIIonbothcellotrioseandinsolublecellulosicsubstrates,suchasAvicel,β-1,3glucan,β-1,4glucan,andPASC.Similarly,cellobiohydrolases,suchasT.reeseiCBHIandII(TrCBHIandII),andtheCBHIfromNeurosporacrassa(NcCBHI)weresuccessfullyexpressedinY.lipolytica. However,theyieldoftheexpressed TrCBHIwaslow,soworkonthiswasnotpursued.Contrastingly,rhNcCBHIwasnotonlywellexpressed,butalsohighlyactiveonPASCandmoreactiveonAvicel(0.11 U/mg)thanwild-type TrCBHI(0.065 U/mg).Therefore,workwaspursuedusingacombinationof NcCBHIand TrCBHII.ThequantificationofenzymelevelsinculturesupernatantsrevealedthattheuseofahybridpromoterinsteadoftheprimarilyusedTEFpromoterprocuredfourandeighttimesmore NcCBHIand TrCBHIIexpressions,respectively.Finally,thecoexpressionofthepreviouslydescribed Y.lipolyticaβ-glucosidases,theCBHII,andEGIandIIfromT.reesei,andtheN.crassaCBHIprocuredanengineered Y.lipolyticastrainthatwasabletogrowbothonmodelcellulosesubstrates,suchashighlycrystallineAvicel,andonindustrialcellulosepulp,suchasthatobtainedusinganorganosolvprocess.Conclusions:A Y.lipolyticastraincoexpressingsixcellulolyticenzymecomponentshasbeensuccessfullydeveloped.Inaddition,theresultspresentedshowhowtherecombinantstraincanbeoptimized,forexample,usingartificialpromoterstotailorexpressionlevels.Mostsignificantly,thisstudyhasprovidedademonstrationofhowthestraincangrowonasampleofindustrialcelluloseassolecarbonsource,thusrevealingthefeasibilityofYarrowia-basedconsolidatedbioprocessfortheproductionoffuelandchemicalprecursors.Further,enzymeandstrainoptimization,coupledtoappropriateprocessdesign,willundoubtedlyleadtomuchbetterperformancesinthefuture.
Immobilizationoftwoendoglucanasesfromdifferentsources.
Sarcina,R.,Giosafatto,C.V.L.,Faraco,V.,Lama,L.,Esposito,M.&Mariniello,L.(2017).InternationalJournalofEnvironment,AgricultureandBiotechnology,2(4),1809-1813.
LinktoArticle
ReadAbstract
Cellulasesareaimportantfamilyofhydrolyticenzymeswhichcatalyzethebondofcelluloseandotherrelatedcello-oligosaccharidederivates.Industrialapplicationsrequireenzymeshighlystableandeconomicallyviableintermsofreusability.Thesecostscanbereducedbyimmobilizingthecellulases,offeringapotentialsolutionthroughenzymerecyclingandeasyrecovery.Thecovalentimmobilizationofenzymesisreportedhere:oneiscommercialcellulasefromAspergillusnigerandotheroneisrecombinantenzyme,namedCelStrepitbecausewasisolatedfromanewcellulolyticstrain,Streptomycessp.G12,.TheoptimalpHforbindingis4.6forbothcellulasesandtheoptimalenzymeconcentrationsare1mg/mLand5mg/mLrespectively.Thesupportforimmobilizationisapoliacrylicmatrix.Experimentscarriedoutinthisworkshowpositiveresultsofenzymeimmobilizationintermsofefficiencyandstabilityandconfirmtheeconomicandbiotechnicaladvantagesofenzymeimmobilizationforawiderangeofindustrialapplications.
ChemicalcharacterizationandimmunomodulatoryactivityofacetylatedpolysaccharidesfromDendrobiumdevonianum.
Deng,Y.,Li,M.,Chen,L.X.,Chen,X.Q.,Lu,J.H.,Zhao,J.&Li,S.P.(2017).CarbohydratePolymers,180,238-245.
LinktoArticle
ReadAbstract
Thechainconformation,chemicalcharactersandimmunomodulatoryactivityofpolysaccharidefromDendrobiumdevonianum(DDP)wereinvestigated.Resultsshowedthatmolecularweights,polydispersityindex,radiusofgyrationsofDDPwere3.99×105 Da,1.27,74.1nm,respectively.Byapplyingthepolymersolutiontheory,theexponent(v)valuesof<>2>z1/2=kMwvwascalculatedas0.38,whichrevealedthatDDPexistedasaglobularshapeinaqueoussolution,andfurtherconfirmedbyAFManalysis.Furthermore,themainmonosaccharidecompositionswereManandGlcwiththeratioof29.61:1.00.Indeed,themainglycosidiclinkageswereβ-1,4-Manp,andsubstitutedwithacetylgroupsatO-2andO-3position.Notably,DDPcouldpromotetheimmunefunctionsofmacrophagesincludingNOreleaseandphagocytosis.Thus,DDPcouldbeexploredasanaturalimmune-stimulatingagentinthehealthandfunctionalfoodareaaswellaspharmaceuticalindustries.
蚂蚁淘电商平台
ebiomall.com
ebiomall.com
公司简介
蚂蚁淘(www.ebiomall.cn)是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台,
该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁
淘搜索全球供应信息,找到合适的产品后在蚂蚁淘下单,然后蚂蚁淘的海外买手进行跨境采购、
运输到中国口岸,最后由蚂蚁淘国内团队报关运输给客户...
蚂蚁淘承诺
正品保证: 全球直采 在线追溯
蚂蚁淘所有产品都是自运营的,我们已经跟国外多家厂方建立品牌推广合作关系, 获得对方的支持和授权; 同时客户可以通过订单详情查看到货物从厂方至客户的所有流程, 确保货物的来源; 正规报关,提供13%增值税发票。
及时交付: 限时必达 畅选无忧
蚂蚁淘的运营团队都是有着多年经验的成员,他们熟悉海外采购、仓储物流、报关等环节; 同时通过在线的流程监控,蚂蚁淘的进口速度比传统企业提高了50%以上, 部分产品甚至能做到7-10天到货,即蚂蚁淘的“时必达”服务。
轻松采购: 在线下单 简单省事
蚂蚁淘的价格是真实透明的,并且具有很大的价格优势,不需要繁杂的询价比价; 报价单与合同可以直接在线生成或打印;就像在京东购物一样, 您的鼠标点击几 次即完成在蚂蚁淘的采购,订单详情会告诉您所有进程。
售后申请: 耐心讲解 优质服务
蚂蚁淘提供的产品在使用过程中如因产品质量问题有售后需求时, 您可通过我的订单提交您的“申请售后”, 蚂蚁淘产品顾问会第一时间为您处理, 在售后服务过程中如遇到问题也可致电蚂蚁淘客服热线:4000-520-616。
磷酸化修饰是一种重要的蛋白翻译后修饰,与信号转导、细胞周期、生长发育以及癌症机理等诸多生物学问题密切相关。鉴定蛋白质磷酸化位点有助于阐明蛋白质磷酸化的机制与生物学功能。本公司合作客户中科院邱金龙老师课题组在 The plant cell 杂志在线发表文章「在拟南芥中由 MPK4 介导的 MYB75 磷酸化是光诱导花青素积累所必需的」。影响因子:8.7。应用背景:为最主要的环境信号之一,光影响植物的多个生理和代谢过程。目前,对植物光受... 查看更多
>
2021-07-16
辣根过氧化酶使试剂中的发光物(luminol)氧化并发光,而试剂中含有增强剂这使得发光增强了1000倍。在免疫印迹中,将复杂的蛋白混合物经sds-page分离,并转移到固相膜上(如nc、pvdf)等,用于免疫学检测,经HRP标记的抗体与膜上的蛋白直接(标记一抗)或间接(标记二抗)反应。... 查看更多
>
货号:CDX-M0113-G002 查看更多
>
2018-03-20
上海冠东生物科技有限公司在发布的SPECTROFLUOR™ Factor Xa fluorogenic substrate荧光发色底物供应信息,浏览与SPECTROFLUOR™ Factor Xa fluorogenic substrate荧光发色底物相关的产品或在搜索更多与SPECTROFLUOR™ Factor Xa fluorogenic substrate荧光发色底物相关的内容。 查看更多
>
嘉美生物是一家主要从事Annexin V,信号转导抗体,重组人和动物蛋白,磷酸化抗体,各种动物ELISA试剂盒,生物实验服务等研发与销售的高科技生物公司,免费热线:400-698-4168。 查看更多
>
2021-08-19
上海甄准生物科技有限公司在发布的不饱和脂肪醇醋酸酯标准品--上海甄准供应信息,浏览与不饱和脂肪醇醋酸酯标准品--上海甄准相关的产品或在搜索更多与不饱和脂肪醇醋酸酯标准品--上海甄准相关的内容。 查看更多
>
2021-07-21
HTI (Haemaologic Technologies)发色底物 优势订购,量大优惠,,HTI (Haemaologic Technologies)发色底物 优势订购,量大优惠, 查看更多
>
2021-07-23
上海研生实业有限公司所提供的IRS-2 胰岛素受体底物-2(抗原)质量可靠、规格齐全,上海研生实业有限公司不仅具有精湛的技术水平,更有良好的售后服务和优质的解决方案,欢迎您来电咨询此产品具体参数及价格等详细信息! 查看更多
>
2018-11-20
建立了贸易往来的品牌有Qiagen,Roche,Miltenyi,Omega,lifespan,Novus,Biomiga,chromotek,chondrex, Sigma, Invitrogen, Abcam, Santa cruz,ebioscienc... 查看更多
>
2021-07-17
天根 可溶型单组分TMB底物溶液
规格(核心参数):5×100ml 查看更多
>
2018-11-08
西宝生物提供多种淀粉酶底物(麦芽七糖苷和麦芽三糖苷),适用于不同方法的诊断试剂盒,用于检测血清和尿液中的淀粉酶。品种齐全,稳定性好,并经过多家诊断试剂盒生产厂家的认证,详情致电400-021-8158!检测用底物及测定原理Alpha淀粉酶底物可分为两类1)麦芽七糖苷,水解产物为对**苯酚(PNP),代表产品有:4,6-亚乙基-对**苯-α-D-麦芽七糖苷4,6-苄基-对**苯-α-D-麦芽七糖苷在alpha淀粉酶... 查看更多
>
2021-07-22
蚂蚁淘生物科技有限公司作为一家专业性的生物科技公司,主要业务是为科研院所、高等院校、环境保护部门的相关实验室和制药、食品、化工等企业的实验室提供服务。代理经营产品主要包括国内外仪器设备、试剂和消耗品等。自公司成立以来,蚂蚁淘一贯以“正品极速”得到广大新老客户的肯定和支持,我们还将不断提升自身的专业技术水平和服务质量,为您提供更好的 查看更多
>
常见问题
蚂蚁淘所售产品均为正品吗?
蚂蚁淘的创始人兼CEO是钟定松先生,具有十年的从业经验,在业界享有良好的口碑;
Ebiomall是跨境直采平台,我们直接从厂家采购,自己的团队负责国际物流和清关,中间没有第三方,蚂蚁淘承诺所售产品仅为正品,假一罚十。
下单后可以修改订单吗?
未确认状态的订单可以修改,打开“订单详情”页面,点击右上角的“修改订单”即可,若已审核确定,则订单无法修改。
商品几天可以发货?
现货产品付款审核后即可发货,大部分期货产品在3周左右即可到货,提供时必达服务的产品订单审核十天内即可发货。
订单如何取消?
如订单处于未确定状态,进入“我的订单"页面,找到要取消的订单,点击“取消订单”按钮。
可以开发票吗?
本网站所售商品都是正规清关,均开具13%正规发票,发票金额含配送费金额,另有说明的除外。
如何联系商家?
蚂蚁淘任何页面都有在线咨询功能,点击“联系客服”、“咨询”或“在线咨询”按钮,均可咨询蚂蚁淘在线客服人员,
或拨打4000-520-616,除此之外客户可在 联系我们页面找到更多的联系方式。
收到的商品少了/发错了怎么办?
同个订单购买多个商品可能会分为一个以上包裹发出,可能不会同时送达,建议查看订单详情是否是部分发货状态;如未收到,可联系在线客服或者致电4000-520-616。
退换货/维修需要多长时间?
一般情况下,退货处理周期为客户收到产品一个月内(以快递公司显示签收时间为准),包装规格、数量、品种不符,外观毁损、短缺或缺陷,请在收到货24小时内申请退换货;特殊商品以合同条款为准。
商品咨询
不同酶的km_何谓km值?有何意义?一个酶有多种底物时,如何判断其...123
2018-03-29
Km值等于酶促反应速度达到最大反应速度一半时所对应的底物浓度,是酶的特征常数之一。
不同的酶Km值不同,同一种酶与不同底物反应Km值也不同,Km值可近似的反应酶与底物的亲和力大小:Km值大,表明亲和力小;Km值小,表明亲合力大。
Km最小的那个底物,就是酶的最适底物。
不同的酶Km值不同,同一种酶与不同底物反应Km值也不同,Km值可近似的反应酶与底物的亲和力大小:Km值大,表明亲和力小;Km值小,表明亲合力大。
Km最小的那个底物,就是酶的最适底物。
【讨论】ELISA反应的显色剂TMB显色后放置多久会褪色 临床检验 ...123
shangmu09232021-07-22
ELISA实验,操作的时候,加入底物,溶液变成蓝色,孵育一段时间后,加入终止液,变成黄色,测吸光值。然后,当天忘记扔板子了,第二天想到去扔的时候,所有孔都变成无色透明的了!!!为什么会褪色???是试剂有问题?还是因为室温太高环境影响?如果是试剂问题那我之前做的结果还能信么?
反应物和底物是不是一个概念? 123
2021-07-27
不是
底物浓度的含义?底物浓度的含义是什么?麻烦再举个例子._123
2018-03-25
底物为参与生化反应的物质,可为化学元素、分子或化合物,经酶作用可形成产物。 底物浓度是指实验反应物的浓度 。比如淀粉酶分解淀粉淀粉溶液的浓度就是指底物浓度。
酶与底物形成中间产物有什么意义_123
TMLiYing2021-07-26
酶是不参加反应的,首先要认识这一点。酶的作用是降低反应的活化能,即与底物结合后,能使底物更容易反应。那么酶是如何行成中间底物的呢?我们知道酶是有专一性的,可以比喻酶是开门的钥匙,锁是底物,拿钥匙去开门这一结合就产生了中间产物。(我已多一年多不学生物,科学需要严谨,请多多参考课本)
[求助]:如何提高转化反应中的底物浓度!!!急急 微生物学和寄生虫学...123
lvtengfei822021-08-10
大家好:
我是新手,来到丁香园看到有这么多的热心人,感到很高兴!!我也有很多问题需要大家的帮助!!实验已经作了一年了可是毫无进展,心里很是着急!!
我的课题是以外消旋的苯基乙二醇为底物,用假丝酵母催化生成手性纯的S-型苯基乙二醇,由于是老课题,所以目标是提高转化反应的底物浓度和菌体的使用批次(目前菌体使用一批后便不能再使用)。
我曾试过很多种方法,但均效果不大!我试着在转化过程中添加醛类,酮类,醇类作为辅助底物,增加菌体的使用批次。可效果不好,特别是添加了醇类后还有的起了反作用,因为我的这个转化过程中涉及到NAD和NADPH的再生。(其转化过程是酵母先催化将外消旋的苯基乙二醇变为酮,再将酮还原为醇,经过这一过程就将外消旋的苯基乙二醇变为手性纯的S-型了)
我还试过用固定化的方法,海藻酸钙包埋法,可是这样底物浓度就更低了!!
我现在不知道下一步该如何做了,很着急,请大家帮帮忙。谢谢了!!谢谢
我是新手,来到丁香园看到有这么多的热心人,感到很高兴!!我也有很多问题需要大家的帮助!!实验已经作了一年了可是毫无进展,心里很是着急!!
我的课题是以外消旋的苯基乙二醇为底物,用假丝酵母催化生成手性纯的S-型苯基乙二醇,由于是老课题,所以目标是提高转化反应的底物浓度和菌体的使用批次(目前菌体使用一批后便不能再使用)。
我曾试过很多种方法,但均效果不大!我试着在转化过程中添加醛类,酮类,醇类作为辅助底物,增加菌体的使用批次。可效果不好,特别是添加了醇类后还有的起了反作用,因为我的这个转化过程中涉及到NAD和NADPH的再生。(其转化过程是酵母先催化将外消旋的苯基乙二醇变为酮,再将酮还原为醇,经过这一过程就将外消旋的苯基乙二醇变为手性纯的S-型了)
我还试过用固定化的方法,海藻酸钙包埋法,可是这样底物浓度就更低了!!
我现在不知道下一步该如何做了,很着急,请大家帮帮忙。谢谢了!!谢谢
【反应底物=反应物?生物中酶与ATP是否算做反应底物中?】123
love123tyj2018-03-29
不算,酶作用于反应底物,ATP提供能量
DNA没有被限制性内切酶切开或者切割不完全怎么办? 123
txstbbm2021-08-11
可以考虑以下几种情况:
1,底物DNA上没有该限制酶的识别、切断位点。特别是一些经过重组等处理的DNA,碱基易发生缺失、变化等。
2,限制酶识别位点上的A或C被甲基化。部分限制酶对识别位点中的碱基是否被甲基化比较敏感,从而无法切断该位点。
3,底物不纯。如果底物DNA中有限制酶阻害物质,回影响限制酶的酶切作用。在此种情况下,底物DNA须重新进行精制。
4,限制酶的识别、切断位点在底物DNA的高级构造中所处的位置,对酶切反应也有一定的影响,例如,限制酶NaeI在切断pBR322DNA时,就有着非常难以切断的部位。
5,限制性内切酶本身无活性或低活性
1,底物DNA上没有该限制酶的识别、切断位点。特别是一些经过重组等处理的DNA,碱基易发生缺失、变化等。
2,限制酶识别位点上的A或C被甲基化。部分限制酶对识别位点中的碱基是否被甲基化比较敏感,从而无法切断该位点。
3,底物不纯。如果底物DNA中有限制酶阻害物质,回影响限制酶的酶切作用。在此种情况下,底物DNA须重新进行精制。
4,限制酶的识别、切断位点在底物DNA的高级构造中所处的位置,对酶切反应也有一定的影响,例如,限制酶NaeI在切断pBR322DNA时,就有着非常难以切断的部位。
5,限制性内切酶本身无活性或低活性
底物是什么_123
吴文昊埼2018-06-12
底物就是参与反应的物质,就像化学反应中的反应物。比如,蛋白酶的底物就是蛋白质、脂酶的底物就是脂类、淀粉酶的底物就是淀粉。
30例ALT严重底物耗尽现象的探讨123
子汉2021-08-19
相关疾病:肝炎肾衰竭休克今天遇到1ICU病人病史是休克肾功不全审生化单时遇到一情况ALT与AST结果与2天前的结果差异极大查看生化反应曲线,如下图很明显是底物耗尽的图,需要稀释,用生理......
酶在酶促反应中能催化特定的底物反应.与酶的有关.酶促反应的速率...123
2021-08-03
酶能成为酶促反应的底物吗?
【反应底物】123
虚幻の梦想2021-08-13
生物中所谓的反应底物是什么?就是化学中的反应物吗?
▍
品牌问答
暂无品牌问答