Ni-NTA XPure Agarose Resin can be used to purify 6xHis-tagged proteins expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells. Ni-NTA XPure Agarose Resin consists of 90µm beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule. The structure of Ni-NTA is compatible with a certain concentration of reducing agents, denaturing agents, detergents and other additives. Ni-NTA XPure Agarose Resin is highly stable and expand the range of suitable operating conditions.

Additional Information

Product Name: Catalog No.: Size: Bead (Geometry, size): Cross-Linked: Ligand: Agarose %: Binding Capacity: Volume %: Storage Temperature: Application: PDF Data Sheet: Users Manual: Image(s): Shipping Method:

Ni-NTA XPure Agarose Resin

P3020-25

25 ml

45 - 165 µm

Yes

Nitilotriacetic acid (NTA)

Highly cross-linked 6% agarose

> 40mg 6xHis-tagged protein/ml medium

50% (v/v) aqueous suspension containing 20% Ethanol

2-8 °C

Batch, Gravity, MPLC and FPLC

PDF Datasheet

PDF User’s Guide

No

Wet ice shipping

Details

Ni-NTA XPure Agarose Resin can be used to purify 6xHis-tagged proteins expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells. Ni-NTA XPure Agarose Resin consists of 90µm beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule. The structure of Ni-NTA is compatible with a certain concentration of reducing agents, denaturing agents, detergents and other additives. Ni-NTA XPure Agarose Resin is highly stable and expand the range of suitable operating conditions. The XPure series of resins are made for large scale and fine purification using a high-performace liquid chromatography (HPLC) system.