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eEnzyme是一家美国高质量生物学试剂及检测试剂盒品牌供应商,其与美国国立卫生研究院合作紧密,在全球范围内为广大科研客户提供高品质的产品与技术服务,其主要生产销售高质量的抗体和用于传染病研究的抗体/抗原,细胞因子蛋白,pcr产品等用于分子和细胞生物学研究的生物试剂,产品应用于生物技术研究,制药,药物发现,癌症研究,传染性疾病,微生物和分子诊断等众多生命科学技术领域
eENZYME为科学界生产和销售高质量的生物试剂盒和生物分析试剂盒。与NIH密切合作以保证产品在生命科学研究的多个领域成功地使用。努力提供新颖价廉且有效的工具,使实验更容易成功。产品在科研、生物技术、制药和政府的研究实验室中得到广泛的应用,包括药物发现、癌症研究、传染病研究、微生物学和分子诊断。
【简单介绍】eENZYME生产销售高质量的抗体和用于传染病研究的抗体/抗原,细胞因子蛋白,PCR产品等用于分子和细胞生物学研究的生物试剂,产品应用于生物技术研究,制药,药物发现,癌症研究,传染性疾病,微生物和分子诊断等众多生命科学技术领域。【详细说明】欢迎eENZYMEeENZYME生产销售高质量的抗体和用于传染病研究的抗体/抗原,细胞因子蛋白,PCR产品等用于分子和细胞生物学研究的生物试剂,产品应用于生物技术研究,制药,药物发现,癌症研究,传染性疾病,微生物和分子诊断等众多生命科学技术领域。 Ë ENZYMEis的高品质生物试剂及实验室设备科学界一个制造商和分销商。我们密切合作,与美国国立卫生研究院的社区,以保证我们的产品生命科学研究在广泛领域的成功表现。我们致力于提供创新和具有成本效益的工具,使您更容易取得成功。我们的产品用于在学术,生物技术,制药和政府研究实验室,包括药物发现,癌症研究,传染病研究,微生物学和分子诊断许多不同的应用。公司网站:www.eenzyme.com 目录号描述产品类型应用单位供应商IA-01SW-0100抗HA(A/California/06/09)(甲型H1N1流感)兔多克隆抗体WB等100微克eENZYMEIA-011-0100抗H1(H1N1/Pan)兔多克隆抗体WB等100微克eENZYMEIA-001-0100抗HA1(A/新喀里多尼亚)(甲型H1N1流感)兔多克隆抗体WB等100微克eENZYMEIA-023-0100抗-NP(A/新Caledonia/20/1999)(甲型H1N1流感兔多克隆抗体WB等100微克eENZYMEIA-022-0100抗M2型(H1N1/A/新喀里多尼亚(H1N1))兔多克隆抗体WB等100微克eENZYMEIA-004A-0100抗-H3(H3N2)(A/WIS/2005)兔多克隆抗体WB等100微克eENZYMEIA-003-0100抗-M1(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-004-0100抗HA(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-020-0100抗-NA(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-005H-0100抗H5(A/香港Kong/483/97)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-005-0100抗HA1(H5N1)(A/Vietnam/1203/2004)兔多克隆抗体WB等100微克eENZYMEIA-006-0100抗HA1(H5N1,禽流感)(A/斑头雁/Qinghai/12/05)兔多克隆抗体WB等100微克eENZYMEIA-010-0100抗-HA2(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-012-0100抗-M1(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-021-0100抗-NA(A/Vietnam/1203/2004)(H5N1,人)兔多克隆抗体WB等100微克eENZYMEIA-025-0100抗NS1(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-003-0100抗HA1(A/Chicken/NY/29878/91)(H2N2)兔多克隆抗体WB等100微克eENZYMEIA-009-0100抗HA1(A/chicken/HongKong/NT366/03)(H9N2)兔多克隆抗体WB等100微克eENZYMEIA-027-0100抗-NA(A/Chicken/HongKong/NT366/03)(H9N2)兔多克隆抗体WB等100微克eENZYMEIA-007-0100抗HA1(A/duck/Hainan/6/2004)(H6N2)兔多克隆抗体WB等100微克eENZYMEIA-008-0100抗HA1(A/鸡/荷兰/2/03)兔多克隆抗体WB等100微克eENZYMEIA-028-0100抗-NA(A/Chicken/Netherlands/1/03)(H7N7)兔多克隆抗体WB等100微克eENZYMEIA-0015-0100抗-H11(A/duck/Yangzhou/906/02)(H11N2)兔多克隆抗体WB等100微克eENZYMEIA-003-0031抗B型流感(NP)的多克隆兔多克隆抗体WB等100微克eENZYMEIA-003-0032抗B型流感(医管局)多克隆兔多克隆抗体WB等100微克eENZYMEIA-013-0100抗-H12(A/pintail/Alaska/102/05)(H12N5)兔多克隆抗体WB等100微克eENZYMEIA-014-0100抗-H4(A/environment/Maryland/1101/06)(H4N6)兔多克隆抗体WB等100微克eENZYMEIA-003-018抗-H15(A/Australia/83/00(H15N8))兔多克隆抗体WB等100微克eENZYMEIA-003-019抗-H16(A/Sweden/99(H16N3))兔多克隆抗体WB等100微克eENZYME目录号描述产品类型应用单位供应商IA-01SW-0100抗HA(A/California/06/09)(甲型H1N1流感)兔多克隆抗体WB等100微克eENZYMEIA-011-0100抗H1(H1N1/Pan)兔多克隆抗体WB等100微克eENZYMEIA-001-0100抗HA1(A/新喀里多尼亚)(甲型H1N1流感)兔多克隆抗体WB等100微克eENZYMEIA-023-0100抗-NP(A/新Caledonia/20/1999)(甲型H1N1流感兔多克隆抗体WB等100微克eENZYMEIA-022-0100抗M2型(H1N1/A/新喀里多尼亚(H1N1))兔多克隆抗体WB等100微克eENZYMEIA-004A-0100抗-H3(H3N2)(A/WIS/2005)兔多克隆抗体WB等100微克eENZYMEIA-003-0100抗-M1(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-004-0100抗HA(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-020-0100抗-NA(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-005H-0100抗H5(A/香港Kong/483/97)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-005-0100抗HA1(H5N1)(A/Vietnam/1203/2004)兔多克隆抗体WB等100微克eENZYMEIA-006-0100抗HA1(H5N1,禽流感)(A/斑头雁/Qinghai/12/05)兔多克隆抗体WB等100微克eENZYMEIA-010-0100抗-HA2(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-012-0100抗-M1(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-021-0100抗-NA(A/Vietnam/1203/2004)(H5N1,人)兔多克隆抗体WB等100微克eENZYMEIA-025-0100抗NS1(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-003-0100抗HA1(A/Chicken/NY/29878/91)(H2N2)兔多克隆抗体WB等100微克eENZYMEIA-009-0100抗HA1(A/chicken/HongKong/NT366/03)(H9N2)兔多克隆抗体WB等100微克eENZYMEIA-027-0100抗-NA(A/Chicken/HongKong/NT366/03)(H9N2)兔多克隆抗体WB等100微克eENZYMEIA-007-0100抗HA1(A/duck/Hainan/6/2004)(H6N2)兔多克隆抗体WB等100微克eENZYMEIA-008-0100抗HA1(A/鸡/荷兰/2/03)兔多克隆抗体WB等100微克eENZYMEIA-028-0100抗-NA(A/Chicken/Netherlands/1/03)(H7N7)兔多克隆抗体WB等100微克eENZYMEIA-0015-0100抗-H11(A/duck/Yangzhou/906/02)(H11N2)兔多克隆抗体WB等100微克eENZYMEIA-003-0031抗B型流感(NP)的多克隆兔多克隆抗体WB等100微克eENZYMEIA-003-0032抗B型流感(医管局)多克隆兔多克隆抗体WB等100微克eENZYMEIA-013-0100抗-H12(A/pintail/Alaska/102/05)(H12N5)兔多克隆抗体WB等100微克eENZYMEIA-014-0100抗-H4(A/environment/Maryland/1101/06)(H4N6)兔多克隆抗体WB等100微克eENZYMEIA-003-018抗-H15(A/Australia/83/00(H15N8))兔多克隆抗体WB等100微克eENZYMEIA-003-019抗-H16(A/Sweden/99(H16N3))兔多克隆抗体WB等100微克eENZYME目录号描述产品类型应用单位供应商IA-01SW-0100抗HA(A/California/06/09)(甲型H1N1流感)兔多克隆抗体WB等100微克eENZYMEIA-011-0100抗H1(H1N1/Pan)兔多克隆抗体WB等100微克eENZYMEIA-001-0100抗HA1(A/新喀里多尼亚)(甲型H1N1流感)兔多克隆抗体WB等100微克eENZYMEIA-023-0100抗-NP(A/新Caledonia/20/1999)(甲型H1N1流感兔多克隆抗体WB等100微克eENZYMEIA-022-0100抗M2型(H1N1/A/新喀里多尼亚(H1N1))兔多克隆抗体WB等100微克eENZYMEIA-004A-0100抗-H3(H3N2)(A/WIS/2005)兔多克隆抗体WB等100微克eENZYMEIA-003-0100抗-M1(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-004-0100抗HA(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-020-0100抗-NA(H3N2)[A/Wyoming/3/03)兔多克隆抗体WB等100微克eENZYMEIA-005H-0100抗H5(A/香港Kong/483/97)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-005-0100抗HA1(H5N1)(A/Vietnam/1203/2004)兔多克隆抗体WB等100微克eENZYMEIA-006-0100抗HA1(H5N1,禽流感)(A/斑头雁/Qinghai/12/05)兔多克隆抗体WB等100微克eENZYMEIA-010-0100抗-HA2(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-012-0100抗-M1(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-021-0100抗-NA(A/Vietnam/1203/2004)(H5N1,人)兔多克隆抗体WB等100微克eENZYMEIA-025-0100抗NS1(A/Vietnam/1203/2004)(H5N1)兔多克隆抗体WB等100微克eENZYMEIA-003-0100抗HA1(A/Chicken/NY/29878/91)(H2N2)兔多克隆抗体WB等100微克eENZYMEIA-009-0100抗HA1(A/chicken/HongKong/NT366/03)(H9N2)兔多克隆抗体WB等100微克eENZYMEIA-027-0100抗-NA(A/Chicken/HongKong/NT366/03)(H9N2)兔多克隆抗体WB等100微克eENZYMEIA-007-0100抗HA1(A/duck/Hainan/6/2004)(H6N2)兔多克隆抗体WB等100微克eENZYMEIA-008-0100抗HA1(A/鸡/荷兰/2/03)兔多克隆抗体WB等100微克eENZYMEIA-028-0100抗-NA(A/Chicken/Netherlands/1/03)(H7N7)兔多克隆抗体WB等100微克eENZYMEIA-0015-0100抗-H11(A/duck/Yangzhou/906/02)(H11N2)兔多克隆抗体WB等100微克eENZYMEIA-003-0031抗B型流感(NP)的多克隆兔多克隆抗体WB等100微克eENZYMEIA-003-0032抗B型流感(医管局)多克隆兔多克隆抗体WB等100微克eENZYMEIA-013-0100抗-H12(A/pintail/Alaska/102/05)(H12N5)兔多克隆抗体WB等100微克eENZYMEIA-014-0100抗-H4(A/environment/Maryland/1101/06)(H4N6)兔多克隆抗体WB等100微克eENZYMEIA-003-018抗-H15(A/Australia/83/00(H15N8))兔多克隆抗体WB等100微克eENZYMEIA-003-019抗-H16(A/Sweden/99(H16N3))兔多克隆抗体WB等100微克eENZYME
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先提取RNA,反转录成cDNA,然后根据目的基因设计PCR引物,通过半定量RT-PCR确定目的基因表达.
正因为检测的是mRNA,所以要先反转录成cDNA才能PCR.
① 在构建载体时,目的基因直接整合到细胞染色体组上,最好不要通过先瞬转在筛选稳定细胞株的这种方法,因为转染效率没有保证
② 高表达载体的构建,哺乳动物表达量一直是它自身的缺点,最好根据高表达载体定向的驯化细胞,提高蛋白表达量
③ 细胞的选择,筛选稳定细胞株我们常用的细胞是CHO,中国仓鼠卵巢细胞,由于CHO具有诸多的优点因此适合用于筛选稳定细胞株,而HEK293细胞则常用于瞬时转染
④ 后期的筛选,双抗预防污染,筛选细胞的时候抗生素浓度一定要做预实验,而且转染的时候不能有抗生素,关于细胞转染 稳定细胞系构建的相关理论
最近因工作需要,欲购进一些生产用的细胞株和瞬转、稳转质粒,大家有什么推荐的品系和公司或网站,谢谢!!!
主要需求:
1.生产或生物制药用的,稳定细胞株如CHO-K1、GS和HEK293/HEK293T,以及与之对应的瞬转、稳转(重组整合)质粒(需详细图谱)。
2.原始或改造细胞株,以及对应质粒,改造细胞株请详述细节,原因,优缺点,安全性等。
3.希望大家积极推荐,实验用、生产用均可,原核、真核都行,网站或品系名称也行,只要大家觉得常用、稳定、安全、高产就行。
4.请有意向的同仁回帖或将详细资料发至我163邮箱:wangqiang23mars@163.com,万分感谢。
然后你得确定,这个细胞株对于你得研究来说不可或缺,具有明确的代表性。
上面两个都没有问题的话,祝你顺利。
如果经费充足的话可以找公司包装病毒,如武汉的普健可以提供各种载体的构建及细胞株构建的技术服务。
暂无品牌问答