New Born Bovine Calf Serum (NBSF) is obtained from blood taken from calves aged less than 20 days, deemed fit for human consumption following ante and post mortem veterinary inspection. The blood is collected in slaughter-houses supervised by government veterinarians.
The blood is allowed to clot naturally or manually defibrinated and is then centrifuged to obtain the liquid fraction of the blood which is now regarded as semi-processed serum. No other additions or deletions of material are permitted.
STERILE FILTERED: Semi-processed Raw New Born Bovine Serum, is thawed, pooled and filtered through a series of sterile membranes down to 0.2 microns before being finally packaged, usually in 500ml or 1L bottles. No further processing, additions or deletions are performed.
Specification - New Born Calf Serum - Sterile Filtered (NBSF) | |
Product | New Born Calf Serum - Sterile Filtered |
Catalogue No. | NBSF |
Source | Bovine blood from healthy calves aged less than 20 days old which have been inspected ante and post mortem by Australian or New Zealand Government veterinarians and passed as fit for human consumption. |
Collection/Processing Method | Blood is collected into aseptic containers, defibrinated and centrifuged under temperature controlled conditions. Serum is subsequently passed through a series of filters terminating with 0.2 micron pharmaceutical grade filters. Product is dispensed in ISO 14644 Class 5 Laminar Flow Workstation via a closed system. It is then immediately frozen. |
Description | A clear orange viscous liquid with a characteristic odour. |
Pack Size | 500ml & 1000ml sterile PET & PETG "Nalgene" bottles |
Storage | -20 degrees Centigrade |
Expiry Date | Six years from the date of manufacture |
Test | Method | Specification | ||||||||||||
Visual Examination | Visual | Satisfactory | ||||||||||||
Specificity | Species I.D. | Bovine | ||||||||||||
Sterility | Millipore Steritest Broth Culture 14 Day Incubation | Sterile | ||||||||||||
Mycoplasma | Broth Culture 35 Day Incubation | Not detected | ||||||||||||
Viruses | Modified 9CFR (113-53c) | |||||||||||||
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Antibodies | ||||||||||||||
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pH | pH Meter | 6.9 - 7.9 | ||||||||||||
Osmolality: | Osmometer | 240 - 340 mOsmol/kg | ||||||||||||
Total Protein | Beckman Coulter Synchron Clinical Systems | 50 - 80 mg/ml | ||||||||||||
Albumin | Electrophoresis | As Reported mg/ml | ||||||||||||
Total Globulins | Electrophoresis | As Reported mg/ml | ||||||||||||
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Electrophoresis pattern | Agarose Gel Plate | Typical | ||||||||||||
Haemoglobin | UV/VIS Spectrophotometer | ≤ 0.25 mg/ml | ||||||||||||
Endotoxin | Kinetic - Turbidimetric | ≤ 30 IU/ml |
FUNCTIONALITY TEST | ||||||||||||||
Cell Line Used | ||||||||||||||
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BIOCHEMICAL PROFILE - Beckman DXC 800 Analyser | ||
Sodium | As Reported mmol/l | |
Potassium | As Reported mmol/l | |
Chloride | As Reported mmol/l | |
Bicarbonate | As Reported mmol/l | |
Anion Gap | As Reported mmol/l | |
Glucose | As Reported mmol/l | |
Urea | As Reported mmol/l | |
Creatinine | As Reported µmol/l | |
Urea/Creatinine | As Reported (ratio) | |
Urate | As Reported mmol/l | |
Bilirubin total | As Reported µmol/l | |
Calcium | As Reported mmol/l | |
Calcium (Alb Corr.) | As Reported mmol/l | |
Phosphate | As Reported mmol/l | |
Alkaline Phosphatase | As reported U/l | |
Gamma Glutamyl Transferase | As reported U/l | |
Alanine Transaminase (Glutamic Pyruvic Transaminase) | As reported U/l | |
Aspartate Transaminase (Glutamic Oxaloacetic Transaminase) | As reported U/l | |
Lactate Dehydrogenase | As reported U/l | |
Cholesterol | As Reported mmol/l | |
Triglyceride | As Reported mmol/l |
EP/USP
The European Pharmacopeia consists of a number of general and specific monographs covering various classes of products.
The monographs set out requirements to be met and followed for all products in the class.
Recently the EP has introduced a Monograph for Bovine Serum – Monograph No 04/2006:2262 to be found in E.P. 5.4
This monograph provides a definition of Bovine Serum and sets out details of production, and requirements for viral inactivation parameters, quality control testing, storage conditions and labelling.
Moregate Bovine Serum products meet these requirements and Certificates of Analysis are modeled on the QC test requirements.
The United States Pharmacopeia-National Formulary (USP-NF) contains standards for medicines, dosage forms, drug substances, excipients, medical devices, and dietary supplements.
Within the Pharmacopeia are Monographs and general chapters.
The monographs consist of information such as the ingredient name, definition, packaging and labelling requirements, storage and a specification.
The specifications list tests to be performed along with the procedure to be followed and the acceptable limit.
EMEA/CPMP/CVMP
The European Medicines Agency (EMEA) is a decentralised body of the European Union with headquarters in London. It sits alongside the European Directorate for the Quality of Medicines (EDQM).
The main responsibility is the protection and promotion of public and animal health, through the evaluation and supervision of medicines for human and veterinary use.
The EMEA publishes guidelines on quality, safety and efficacy testing requirements.
These guidelines are prepared by committees, and those guidelines which relate to the use of Bovine Serum, including Fetal Bovine Serum, in the manufacture of medicines include:
CPMP: Committee for Proprietary Medicinal Products
Note for Guidance on the Use of Bovine Serum in the Manufacture of Human Biological Medicinal Products
Note for Guidance on Minimising the Risk of Transmitting Animal Spongiform Encephalopathy Agents via Human and Veterinary Medicinal Products. (Also adopted by the CVMP)
CVMP: Committee for Veterinary Medicinal Products
Requirements and Controls applied to Bovine Serum used in the production of Immunological Veterinary Medicinal products.
These Guidelines include requirements for Virus Testing of Fetal Bovine Serum and other Bovine Serum. Further reference to these requirements can be found in “Virus Testing”.
All Moregate Biotech Fetal Bovine Serum and other Bovine Serum meet the requirements of the Guidelines in relation to Fetal Bovine Serum and Bovine Serum.
USDA 9CFR Part 113.53c
Ingredients of animal origin used in the United States of America, in the manufacture of veterinary and human biologics are required to be in compliance with the Code of Federal Regulations, Title 9 – Animals and Animal Products, Chapter 1 - Animal and Plant Health Inspection Service, Department of Agriculture Part 113-53c, commonly referred to as 9CFR Part 113-53c.
This legislation sets out the requirements for detection of extraneous viruses, detailing the methods to be used and the list of viruses that shall be tested for.
Over time 9CFR Part 113-53c became the accepted standard for the testing of animal sera, particularly Fetal Bovine Serum for adventitious viral agents.
Bovine Respiratory Syncytical Virus, Bovine Viral Diarrhea Virus, Bovine Parvovirus, Bluetongue Virus, Bovine Adenovirus, Rabies Virus and Reovirus are tested for by fluorescent antibody
Infections Bovine Rhinotracheitis tested for by Cytopathic Agents.
Infectious Influenza 3 tested for by Haemadsorbing Agents
Hazards Identification
- Not hazardous
- Get Medical attention immediately.
First Aid Measures
Get Medical attention immediately.
Ingestion
If swallowed, give several glasses of water to drink to dilute.
Skin Contact
Wash skin with soap and copious amounts of water.
Eye Contact
Flush with water for at least 15 minutes, lifting upper and lower eyelids occasionally.
Accidental Release
Procedures for Personal Precaution
Exercise appropriate precautions to minimize direct contact with skin or eyes.
Methods for Cleaning Up
Mop up
Ventilate area and wash spill site after material pickup is complete
Handling and Storage
Handling
Normal measures for preventive fire protection
Storage
Keep tightly closed under correct storage conditions
Exposure Controls / Personal Protection
- Wash thoroughly after handling
- Protective gloves
Disposal Considerations
Contact a licensed professional waste disposal service to dispose of this material.
Transport Information
- Non-hazardous for road transport
- Non-hazardous for sea transport
- Non-hazardous for air transport
Note: The above information is believed to be correct, but shall be used as a guide only.
Disclaimer: For pharmaceutical use only.
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纤维蛋白已被除去(如通过血凝或去纤维蛋白法)的血浆
血液凝固析出的淡黄色透明液体。如将血液自血管内抽出,放入试管中,不加抗凝剂,则凝血反应被激活,血液迅速凝固,形成胶冻。凝血块收缩,其周围所析出之淡黄色透明液体即为血清,也可于凝血后经离心取得。在凝血过程中,纤维蛋白原转变成纤维蛋白块,所以血清中无纤维蛋白原,这一点是与血浆最大的区别。而在凝血反应中,血小板释放出许多物质,各凝血因子也都发生了变化。这些成分都留在血清中并继续发生变化,如凝血酶原变成凝血酶,并随血清存放时间逐渐减少以至消失。这些也都是与血浆区别之处。但大量未参加凝血反应的物质则与血浆基本相同。为避免抗凝剂的干扰,血液中许多化学成分的分析,都以血清为样品。(右图为血清蛋白)
作用:
●提供基本营养物质:氨基酸、维生素、无机物、脂类物质、核酸衍生物等,是细胞生长必须的物质。
●提供激素和各种生长因子:胰岛素、肾上腺皮质激素(氢化可的松、地塞米松)、类固醇激素(雌二醇、睾酮、孕酮)等。生长因子如成纤维细胞生长因子、表皮生长因子、血小板生长因子等。
●提供结合蛋白:结合蛋白作用是携带重要地低分子量物质,如白蛋白携带维生素、脂肪、以及激素等,转铁蛋白携带铁。结合蛋白在细胞代谢过程中起重要作用。
●提供促接触和伸展因子使细胞贴壁免受机械损伤。
●对培养中的细胞起到某些保护作用:有一些细胞,如内皮细胞、骨髓样细胞可以释放蛋白酶,血清中含有抗蛋白酶成分,起到中和作用。这种作用是偶然发现的,现在则有目的的使用血清来终止胰蛋白酶的消化作用。因为胰蛋白酶已经被广泛用于贴壁细胞的消化传代。血清蛋白形成了血清的粘度,可以保护细胞免受机械损伤,特别是在悬浮培养搅拌时,粘度起到重要作用。血清还含有一些微量元素和离子,他们在代谢解毒中起重要作用,如SeO3,硒等。
热门血清品牌:
1.Gibco
【特点】
已通过ISO9001注册认证
通过最高水平的USP无菌性测试,并经过三重0.1μm过滤
具有ISIA可追溯性认证,血清的生产符合最高的可追溯性标准
澳洲来源特别适用于要求低风险的研究应用
购买推荐:http://www.biomart.cn/infosupply/27094281.htm
2.Hyclone
购买推荐:http://www.biomart.cn/infosupply/2520990.htm
【特点】
Sciencell的每一批胎牛血清均经过严格的质控和测试,包括近300种人类原代细胞筛选。胎牛血清经无菌过滤,pH值在7左右,内毒素不超过0.625EU/ml,且无病毒成分。
购买推荐:http://www.biomart.cn/infosupply/8505015.htm
国产血清推荐:
1.杭州四季青
购买推荐:http://www.biomart.cn/infosupply/33827963.htm
2.金源康
【特点】
质控标准:采自健康新生牛,两次0.1um过滤,无支原体;产品质量符合《中国药典》2015版标准。
产品特点:低lgG,低内毒素,促细胞生长优良
应用范围:娇贵细胞培养,种细胞的继代等。
推荐购买:
以下产品限时买二送一
新生牛血清(超级)http://www.biomart.cn/infosupply/20221746.htm
胎牛血清(优极)http://www.biomart.cn/infosupply/20323300.htm
胎牛血清(超级)http://www.biomart.cn/infosupply/25018976.htm
3.CellMax
推荐购买:
限时暑期特惠
优级胎牛血清http://www.biomart.cn/infosupply/14975942.htm
标准胎牛血清http://www.biomart.cn/infosupply/14975944.htm
澳洲特级胎牛血清http://www.biomart.cn/infosupply/14975935.htm
本人做动物实验,每组设7只,需要测定小鼠的相关指标,初次接触有几点不明白
1、测定指标时,是否可以每组7只,每只测定一次,一共测定7次?
2、是否可以每组选择3只,每只测定一次,共3次?
3、是否可以每组选择3只,每只自身平行测定3次,共9次?
4、测定指标时,是否可以前3只鼠测定A指标,后4只鼠测定B指标吗?
请问1、2、3中的三个方法哪种方法是否具有可行性?4中的测定方法是否可以?
血清蛋白是血浆里最丰富的蛋白质。每一个蛋白分子能携带七个脂肪酸分子。这些脂肪酸分子结合在蛋白的缝隙中,它们富含碳的尾部埋藏在里面安全地避开周围的水分子。血清蛋白同样能够携带许多其它的不溶于水的分子。尤其是血清蛋白,能够携带着许多药物分子,比如布洛芬。
正因为血清蛋白是如此普遍地存在于血液中并且如此容易地被提纯,所以它成为科学家最早研究的蛋白质之一。今天,当需要一种蛋白质时,一种来源于牛体内的类似的蛋白在研究中被广泛地使用,这种蛋白叫做牛族血清蛋白或者称作BSA。许多酶在稀溶液中不稳定,解决的办法是加入一些牛族血清蛋白。在试验中它能使酶稳定,且相对地中性,不会影响酶的性质。
1)提供基本营养物质:氨基酸、维生素、无机物、脂类物质、核酸衍生物等,是细胞生长必须的物质。
2)提供结合蛋白:结合蛋白作用是携带重要地低分子量物质,如白蛋白携带维生素、脂肪、以及激素等,转铁蛋白携带铁。结合蛋白在细胞代谢过程中起重要作用。
3)提供激素和各种生长因子:胰岛素、肾上腺皮质激素(氢化可的松、地塞米松)、类固醇激素(雌二醇、睾酮、孕酮)等。生长因子如成纤维细胞生长因子、表皮生长因子、血小板生长因子等。
4)对培养中的细胞起到某些保护作用:有一些细胞,如内皮细胞、骨髓样细胞可以释放蛋白酶,血清中含有抗蛋白酶成分,起到中和作用。这种作用是偶然发现的,现在则有目的的使用血清来终止胰蛋白酶的消化作用。因为胰蛋白酶已经被广泛用于贴壁细胞的消化传代。血清蛋白形成了血清的粘度,可以保护细胞免受机械损伤,特别是在悬浮培养搅拌时,粘度起到重要作用。血清还含有一些微量元素和离子,他们在代谢解毒中起重要作用,如SeO3,硒等
5)提供促接触和伸展因子使细胞贴壁免受机械损伤。
6)血清中有抗体,这是被称作免疫球蛋白的蛋白质,血清可以抗病毒,增强抵抗力血清属于生物制剂。
本人科研小白,研究新生!
课题准备检测血清中骨桥蛋白(OPN)含量
现在计划是先将所有血标本离心取血清先冻存,待所有标本收集齐后再统一做
大概要收集2个月,约70个病例。
现在我的问题就是:1、离心后的血清,如何分装、冻存?(具体条件、流程、用什么管子冻存,是否需要买冻存液等)
2、冻存后如何复苏?(最好有具体流程,具体复苏条件,是否需要买什么复苏液、培养基什么的)
刚来读书,一下子拿到这个题目有点懵,网上搜了一些,比较杂,比较乱,没有底,想请教各位哥哥姐姐给我提供点帮助,先谢谢了!
暂无品牌问答