Low-IgG BSA is a highly purified, lyophilized powder. This product is processed to provide a minimum of 98% albumin and to be essentially free of immunoglobulin. Applications include ELISA and any assay where IgG may interfere.
SPECIFICATION - Bovine Serum Albumin - Low lgG (BSA-LI) | |
Product | Bovine Serum Albumin- Low IgG |
Catalogue No. | BSALI |
Certificate of Suitability | Australia: R1-CEP 2005-191-Rev 00New Zealand: R1-CEP 2003-205-Rev 00 |
Source | Bovine plasma from healthy animals inspected ante and post mortem by New Zealand or Australian Government veterinarians and passed as fit for human consumption |
Collection/Processing Method | Cohn Fraction V Purified by heat shock treatment with charcoal treatment and diafiltrarion. |
Description | Off white to pale green / yellow lyophilised powder |
Stability | Stable for a minimum of 5 years under correct storage conditions |
Storage | Store in sealed containers with a desiccant under dry conditions between 2 - 8°C |
Analysis | Method | Range |
pH | pH meter | 6.5 - 7.2 |
Albumin | Gel Electrophoresis | ≥ 98% |
Protein | LECO CF003.1 (dry basis) | ≥ 96% |
Moisture Content | Oven Moisture Content | ≤ 5.0% |
Chloride | Coulometric | ≤ 0.15% |
Calcium | ICP-OES | ≤ 0.05% |
Iron | ICP-OES | ≤ 0.002% |
Magnesium | ICP-OES | ≤ 0.005% |
Phosphorus, inorganic | Coulometric | ≤ 0.005% |
Potassium | ICP-OES | ≤ 0.015% |
Sodium | ICP-OES | ≤ 0.08% |
Lead | ICP-OES | ≤ 0.003% |
Ash Content | Gravimetric | ≤ 2.5% |
Optical Density | Abs 405nm at 60mg/ml sol | ≤ 0.2 |
Solubility | 60mg/ml sol. | Soluble |
Bioburden | Heterotrophic Colony count - pour plate | ≤ 100 CFU/g |
IgG | Elisa | ≤ 100 µg/g |
EDQM
The European Directorate for the Quality of Medicines (EDQM) has originated from the European Pharmacopoeia Secretariat which, with the addition of new responsibilities changed its name to the European Department for the Quality of Medicines (EDQM). The EDQM sits alongside the European Medicines Agency (EMEA).
Amongst other activities, the EDQM is responsible for the European Pharmacopeia and the issuing of Certificates of Suitability (CEP).
Certificates of Suitability (CEP) are recognised by all signatory states of the European Pharmacopoeia Convention and by the European Union. Other countries have also chosen to recognise them. In the case of Fetal Bovine Serum and other Bovine Serum a CEP can be used by the manufacturers of Fetal Bovine Serum and other Bovine Serum which is intended for use in the manufacture of pharmaceutical products to demonstrate compliance with the Bovine Serum monographs of the European Pharmacopoeia and the EDQM requirements for substances concerned by TSE risk.
The discovery of Bovine Spongiform Encephalopathy (BSE), which is one of a group of similar infections now referred to as Transmissible Spongiform Encephalopathies (TSEs), and it’s spread over many countries of the European Union as well as its discovery in Canada, the USA and Japan is well known. The European events provided the impetus for the Council of Europe Public Health Committee (CEPHC) to pass Resolution AP-CSP (99) 5 addressing the TSE concerns and creating a Certificate of Suitability (COS) pursuant to Directive 75/318/EEC.
The granting of a CoS to a manufacturer, for a particular product, certifies that the product in question has been assessed for the level of risk of transmission of TSEs, and that the risk level is considered low enough that the product is certified as suitable for use in the manufacture of medicinal products in the European Union.
The process of approval requires the manufacturer to submit a dossier that covers all relevant aspects of the collection of raw material and further processing that is performed to reach the product that is offered to end users. This dossier is detailed and covers the specific material that is collected, the collection method, process validation, testing, traceability, quality systems and an expert review.
Only after this dossier has been examined and approved by two rapporteurs and, if necessary, by a panel of experts appointed by the European Directorate for the Quality of Medicines (EDQM) is the manufacturer granted a COS.
Moregate Biotech has been granted a COS for:
- Fetal Bovine Serum – Australian Origin – CEP2000-187
- Fetal Bovine Serum – New Zealand Origin – CEP2000-188
- Adult Bovine Serum - New Zealand Origin – CEP2001-093
- Adult Bovine Serum (Defibrinated) - New Zealand Origin – CEP2000-174
- Bovine Plasma – New Zealand Origin – CEP2003-199
- Bovine Serum Albumin – manufactured in Australia from New Zealand origin Bovine Plasma – CEP2003-205
- Bovine Plasma – Australian Origin - CEP 2005-192
- Bovine Serum Albumin – manufactured in Australia from Australian origin Bovine Plasma - CEP 2005-191
Copies of the Certificates of Suitability are available upon request
Hazards Identification
SPECIAL INDICATION OF HAZARDS TO HUMANS AND THE ENVIRONMENT
- Not hazardous.
First Aid Measures
Get Medical attention immediately.
Inhalation
If inhaled, remove to fresh air. If breathing becomes difficult, call a physician
Ingestion
If swallowed, give several glasses of water to drink to dilute.
Skin Contact
Wash skin with soap and copious amounts of water.
Eye Contact
Flush with water for at least 15 minutes, lifting upper and lower eyelids occasionally.
Procedures for Personal Precaution
Exercise appropriate precautions to minimize direct contact with skin or eyes.
Methods for Cleaning Up
Mop up
Ventilate area and wash spill site after material pickup is complete
Fire fighting Measures
Extinguishing Media
Water spray. Carbon dioxide, dry chemical powder, or appropriate foam.
Special Risks
Emits toxic fumes under fire conditions
Special Protective Equipment for Firefighters
Wear self-contained breathing apparatus and protective clothing to prevent contact with skin and eyes.
Accidental Release
Procedures for Personal Precaution
Exercise appropriate precautions to minimize direct contact with skin or eyes.
Methods for Cleaning Up
Mop up
Ventilate area and wash spill site after material pickup is complete
Handling and Storage
Handling
Avoid contact with eyes, skin and clothing.
Storage
Keep tightly closed. Store at 2 - 8 °C.
Exposure Controls / Personal Protection
- Wash thoroughly after handling.
- Wear dust mask
- Protective gloves
- Chemical safety goggles
Disposal Considerations
Contact a licensed professional waste disposal service to dispose of this material.
Transport Information
- Non-hazardous for road transport
- Non-hazardous for sea transport
- Non-hazardous for air transport
Note: The above information is believed to be correct, but shall be used as a guide only.
Disclaimer: For pharmaceutical use only.
BOVINE SERUM ALBUMIN
Moregate Bovine Serum Albumin BSA is manufactured from Bovine Plasma of Australian or New Zealand origin in a well equipped facility located in Bulimba, Australia. The manufacturing process is a modified Heat Shock method.
The process includes ultrafiltration, micro filtration and freeze-drying.
Trained personnel following Standard Operating Procedures perform all operations. Each batch has a Manufacturing Record, which is reviewed after batch completion.
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纤维蛋白已被除去(如通过血凝或去纤维蛋白法)的血浆
血液凝固析出的淡黄色透明液体。如将血液自血管内抽出,放入试管中,不加抗凝剂,则凝血反应被激活,血液迅速凝固,形成胶冻。凝血块收缩,其周围所析出之淡黄色透明液体即为血清,也可于凝血后经离心取得。在凝血过程中,纤维蛋白原转变成纤维蛋白块,所以血清中无纤维蛋白原,这一点是与血浆最大的区别。而在凝血反应中,血小板释放出许多物质,各凝血因子也都发生了变化。这些成分都留在血清中并继续发生变化,如凝血酶原变成凝血酶,并随血清存放时间逐渐减少以至消失。这些也都是与血浆区别之处。但大量未参加凝血反应的物质则与血浆基本相同。为避免抗凝剂的干扰,血液中许多化学成分的分析,都以血清为样品。(右图为血清蛋白)
作用:
●提供基本营养物质:氨基酸、维生素、无机物、脂类物质、核酸衍生物等,是细胞生长必须的物质。
●提供激素和各种生长因子:胰岛素、肾上腺皮质激素(氢化可的松、地塞米松)、类固醇激素(雌二醇、睾酮、孕酮)等。生长因子如成纤维细胞生长因子、表皮生长因子、血小板生长因子等。
●提供结合蛋白:结合蛋白作用是携带重要地低分子量物质,如白蛋白携带维生素、脂肪、以及激素等,转铁蛋白携带铁。结合蛋白在细胞代谢过程中起重要作用。
●提供促接触和伸展因子使细胞贴壁免受机械损伤。
●对培养中的细胞起到某些保护作用:有一些细胞,如内皮细胞、骨髓样细胞可以释放蛋白酶,血清中含有抗蛋白酶成分,起到中和作用。这种作用是偶然发现的,现在则有目的的使用血清来终止胰蛋白酶的消化作用。因为胰蛋白酶已经被广泛用于贴壁细胞的消化传代。血清蛋白形成了血清的粘度,可以保护细胞免受机械损伤,特别是在悬浮培养搅拌时,粘度起到重要作用。血清还含有一些微量元素和离子,他们在代谢解毒中起重要作用,如SeO3,硒等。
热门血清品牌:
1.Gibco
【特点】
已通过ISO9001注册认证
通过最高水平的USP无菌性测试,并经过三重0.1μm过滤
具有ISIA可追溯性认证,血清的生产符合最高的可追溯性标准
澳洲来源特别适用于要求低风险的研究应用
购买推荐:http://www.biomart.cn/infosupply/27094281.htm
2.Hyclone
购买推荐:http://www.biomart.cn/infosupply/2520990.htm
【特点】
Sciencell的每一批胎牛血清均经过严格的质控和测试,包括近300种人类原代细胞筛选。胎牛血清经无菌过滤,pH值在7左右,内毒素不超过0.625EU/ml,且无病毒成分。
购买推荐:http://www.biomart.cn/infosupply/8505015.htm
国产血清推荐:
1.杭州四季青
购买推荐:http://www.biomart.cn/infosupply/33827963.htm
2.金源康
【特点】
质控标准:采自健康新生牛,两次0.1um过滤,无支原体;产品质量符合《中国药典》2015版标准。
产品特点:低lgG,低内毒素,促细胞生长优良
应用范围:娇贵细胞培养,种细胞的继代等。
推荐购买:
以下产品限时买二送一
新生牛血清(超级)http://www.biomart.cn/infosupply/20221746.htm
胎牛血清(优极)http://www.biomart.cn/infosupply/20323300.htm
胎牛血清(超级)http://www.biomart.cn/infosupply/25018976.htm
3.CellMax
推荐购买:
限时暑期特惠
优级胎牛血清http://www.biomart.cn/infosupply/14975942.htm
标准胎牛血清http://www.biomart.cn/infosupply/14975944.htm
澳洲特级胎牛血清http://www.biomart.cn/infosupply/14975935.htm
本人做动物实验,每组设7只,需要测定小鼠的相关指标,初次接触有几点不明白
1、测定指标时,是否可以每组7只,每只测定一次,一共测定7次?
2、是否可以每组选择3只,每只测定一次,共3次?
3、是否可以每组选择3只,每只自身平行测定3次,共9次?
4、测定指标时,是否可以前3只鼠测定A指标,后4只鼠测定B指标吗?
请问1、2、3中的三个方法哪种方法是否具有可行性?4中的测定方法是否可以?
血清蛋白是血浆里最丰富的蛋白质。每一个蛋白分子能携带七个脂肪酸分子。这些脂肪酸分子结合在蛋白的缝隙中,它们富含碳的尾部埋藏在里面安全地避开周围的水分子。血清蛋白同样能够携带许多其它的不溶于水的分子。尤其是血清蛋白,能够携带着许多药物分子,比如布洛芬。
正因为血清蛋白是如此普遍地存在于血液中并且如此容易地被提纯,所以它成为科学家最早研究的蛋白质之一。今天,当需要一种蛋白质时,一种来源于牛体内的类似的蛋白在研究中被广泛地使用,这种蛋白叫做牛族血清蛋白或者称作BSA。许多酶在稀溶液中不稳定,解决的办法是加入一些牛族血清蛋白。在试验中它能使酶稳定,且相对地中性,不会影响酶的性质。
1)提供基本营养物质:氨基酸、维生素、无机物、脂类物质、核酸衍生物等,是细胞生长必须的物质。
2)提供结合蛋白:结合蛋白作用是携带重要地低分子量物质,如白蛋白携带维生素、脂肪、以及激素等,转铁蛋白携带铁。结合蛋白在细胞代谢过程中起重要作用。
3)提供激素和各种生长因子:胰岛素、肾上腺皮质激素(氢化可的松、地塞米松)、类固醇激素(雌二醇、睾酮、孕酮)等。生长因子如成纤维细胞生长因子、表皮生长因子、血小板生长因子等。
4)对培养中的细胞起到某些保护作用:有一些细胞,如内皮细胞、骨髓样细胞可以释放蛋白酶,血清中含有抗蛋白酶成分,起到中和作用。这种作用是偶然发现的,现在则有目的的使用血清来终止胰蛋白酶的消化作用。因为胰蛋白酶已经被广泛用于贴壁细胞的消化传代。血清蛋白形成了血清的粘度,可以保护细胞免受机械损伤,特别是在悬浮培养搅拌时,粘度起到重要作用。血清还含有一些微量元素和离子,他们在代谢解毒中起重要作用,如SeO3,硒等
5)提供促接触和伸展因子使细胞贴壁免受机械损伤。
6)血清中有抗体,这是被称作免疫球蛋白的蛋白质,血清可以抗病毒,增强抵抗力血清属于生物制剂。
本人科研小白,研究新生!
课题准备检测血清中骨桥蛋白(OPN)含量
现在计划是先将所有血标本离心取血清先冻存,待所有标本收集齐后再统一做
大概要收集2个月,约70个病例。
现在我的问题就是:1、离心后的血清,如何分装、冻存?(具体条件、流程、用什么管子冻存,是否需要买冻存液等)
2、冻存后如何复苏?(最好有具体流程,具体复苏条件,是否需要买什么复苏液、培养基什么的)
刚来读书,一下子拿到这个题目有点懵,网上搜了一些,比较杂,比较乱,没有底,想请教各位哥哥姐姐给我提供点帮助,先谢谢了!
暂无品牌问答