The reticular fiber silver stain is commonly used to demonstrate reticular fibers. The method developed by Gordon and Sweet made further improvement on the reticulin silver stain. However, these reticulin staining procedures use relatively large volume of staining solutions, are costly, time-consuming, and difficult to achieve consistent results.Hito Reticulin OptimStain™ PAP-Pen Kit offers a simple solution to these problems. This kit is easy to use with simplified procedures. The users can process slides in small quantities of solutions . The staining step can be controlled slide by slide to achieve optimal staining and differentiation for each slide. This kit delivers stable and improved staining quality, with minimal overstains, background and artifacts when used properly.Hito Reticulin OptimStain™ PAP-Pen Kit has been tested on the liver, spleen, pancreas, lung and other tissues from several species of animals and proven to be sensitive for demonstrating the morphological details of reticular fibers. It is a simple solution for your research.
Kit Contents | for 50 Slides |
Solution-1 | 20 ml |
Solution-2 | 20 ml |
Solution-3 | 20 ml |
Solution-4A | 10 ml |
Solution-4B | 10 ml |
Solution-4C | 10 ml |
Solution-5 | 20 ml |
Solution-6 | 20 ml |
Solution-7 | 20 ml |
Solution-8 | 20 ml |
Hito Aqua Barrier PAP Pen | 1 |
User Manual and MSDS | 1 |
Before using Hito Reticulin OptimStain™ PAP-Pen Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit): Cryostat or Microtome, Light microscopeParaffin embedding equipment (or paraffin sections)Hito Bouin’s Plus Solution (Cat# HTSH0102, for paraffin sections preparation)Dry ice, isopentane, O.C.T. compound (for frozen sections), 4% PFA (Cat# HTSH0101), ethanol, xylene, double distilled or deionized waterSlides, coverslipsStaining jars for slides washResinous mounting medium Hito Reticulin OptimStain™ PAP-Pen Kit Manual and MSDS |
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1. Buffer中离子浓度过大,甘氨酸或者Tris碱有可能疏忽多加了
2.没有加相应浓度的SDS
3.电泳周围温度高,也是一个原因
加样缓冲液的主要作用是使PCR产物与其混合,使DNA沉于加样孔的底部,防止DNA跑出来.
如果你是制胶或者做电泳缓冲液的话,不用灭菌。
低离子强度时,迁移率快。但离子强度过低,缓冲液的缓冲容量小,不易维持pH恒定。高离子强度时迁移率慢,因此为了获得更快的反应速度,在缓冲容量允许的范围内,离子强度应该尽可能小。
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