Product Name:MxSafe(Security nucleic acid dye)
Catalog Number: Mx007
HeLa cells wereincubated at 37 oC with 1X of SYBR Green I, MxSafe and MxSafe Plus,respectively.Images were taken
following incubation for5 min (panel A) and 30 min (panelB), respectively.SYBR Green I entered into cells rapidly as ev
-ident from thebright green nuclear staining.However, MxSafe and MxSafe Plus were unable tocross cell membranes
as shown by the lack of any fluorescence staining.
MxSafe is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace
thehighly toxicethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. MxSafe
is farmore sensitivethan EB without requiring a destaining step. MxSafeand EB have virtually the same spectra, so you
can directlyreplace EBwithMxSafewithout changing your existing imaging system.
MxSafe is a very stable dye. Store 10,000X solution and dilute solutions ofMxSafe at room temperature, protected
from light. Dye precipitation may occur at lower temperatures, resulting in lower signal or the appearance of
precipitate onthe surface of the gel. If this occurs, heat the solution to 45-50oC for two minutesand vortex.
MxSafeis stable for at least one year from the date it is received.
ebiomall.com
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我在一些文献上看到说,要加入tRNA as carrier,但是这句话不好理解
有没有人用过,跟我讲讲tRNA到底有什么用
多谢指教
1、测浓度和纯度,是否达标
2、跑电泳看条带是否与测的数据相互印证
如果上述两点满足,就不需要纯化,否则需要进一步纯化。
不过根据经验,一般都不需要,我们实验室用的是GNT系列的试剂盒,提取结果就直接进入下游实验了,效果挺稳定
质粒是真核细胞细胞核外或原核生物拟核区外能够进行自主复制的遗传单位,包括真核生物的细胞器(主要指线粒体和叶绿体)中和细菌细胞拟核区以外的环状脱氧核糖核酸(DNA)分子.现在习惯上用来专指细菌(大肠杆菌)、酵母菌和放线菌等生物中细胞核或拟核中的DNA以外的DNA分子.
小鼠没有叶绿体,你可以直接参照小鼠线粒体的提取方法进行提取.
向左转|向右转
植物材料 用最精确的方法,称取不超过100mg的植物材料,置于处理过的研钵,加入液氮进行研磨 将研磨得到的粉末,快速转移至无RNase,并经过液氮冷却的2mL离心管(自备),
目的掌握RNA提取的基本技术,了解RNA提取过程中的各种注意事项。二、原理RNA提取是分子生物学实验中难度较大的实验技术。RNA提取和DNA提取有类似的地方,因为它们都是核酸,都具有较好的水溶性。提取RNA首先破碎细胞,然后用提取液将RNA溶出,反复抽提去除蛋白质 ……
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