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TestedandCertifiedforinvivoimaging(See"LuciferinFAQ"inadditionalinformation)
Luciferinisacommonbioluminescentreporterusedforinvivoimagingoftheexpressionofluciferase.ThiswatersolublesubstrateforthefireflyluciferaseenzymeutilizesATPandMg2+ascofactorstoemitacharacteristicyellow-greenemissioninthepresenceofoxygen,whichshiftstoredlightinvivoat37°C.ThroughtheutilizationofATP,thereactioncanbefurtherusedtoindicatethepresenceofenergyorlifeinordertofunctionasalife-deathstain.
Luciferinisacommonreagentusedthroughoutthebiotechnologyfieldandspecificallyforinvivoimaging.Luciferaselabeledtumorcells,stemcellsorinfectiousdiseasesareofteninoculatedintoresearchanimalssuchasratsormiceforinvestigation.Theinjectionofluciferinallowsforthereal-time,noninvasivemonitoringofdiseaseprogressionand/ordrugefficacyinthesemodelsystemsthroughBioluminescenceImaging(BLI).
Luciferinisalsocommonlyusedforinvitroresearch,includingluciferaseandATPassays,genereporterassays,highthroughputsequencingandvariouscontaminationassays.
FireflyLuciferinisidenticaltoBeetleLuciferin.(See"AdditionalInformation"forstructures.)
ProductSpecifications:
D-Luciferin,PotassiumSalt,
4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylicacidpotassiumsalt,
MOLECULARBIOLOGYGRADE(PremiumPure)
Formula:KC11H7N2O3S2
MW:318.42g/mol
Color:Lightyellowpowder
Purity:>99%pure.(QualityverifiedbynineindependentcriteriaincludingHPLCandFTIR.)
Storage/Handling:Storedesiccatedat-20°C.Protectfromlight.
PubChemChemicalID:44134804