Lyophosome™-Natural Phosphatidylserine (PS) extract catalog is composed of four liposomes made from phosphatidylserine (PS) lipids that are made from natural lipids that are extracted from either brain or soybean. Adding 30% cholesterol makes the liposomes serum stable and is necessary when the liposomes are used in the presence of serum.
These liposomes are all anionic due to the negative charge of phosphatidylserine.
Brain phosphatidylserine (PS) is composed of both saturated and unsaturated lipids. Brain PS contains much larger percentage of saturated lipids compare to Soy PS. Brain PS is mainly composed of four lipids: 42% of 18:0 lipid, 30% of 18:1 lipid, 2% of 20:4 lipid and 11% of 22:6 lipid.
Soy PS is composed of both saturated and unsaturated lipids. Soy PS contains more unsaturated lipids. Soy PC is mainly composed of 5 lipids: 10% of 16:0 lipid, 3% of 18:0 lipid, 12% of 18:1 lipid, 68% of 18:2 lipid, and 7% of 18:3 lipid.
Lyophosome™ product catalog is composed of a large selection of freeze-dried liposomes with various types of lipids and wide range of zeta potentials and different properties. Lyophosome™ products should be used by scientists who understand liposome formulation and have the proper equipment to check the size, separate non-encapsulated drugs and do the proper assays. Freeze-dried liposomes cannot be used blindly.
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1、如果是提取的总蛋白,然后做WB,用β-actin或者GAPDH做内参肯定是没有问题的,这是公认的东西。
2、如果用膜蛋白提取试剂盒提取蛋白,再用β-actin作为内参似乎不妥,因为理论上来讲β-actin在膜上是不表达的。WB能做出β-actin来是因为膜蛋白提取时把胞质蛋白也提出来了。然而,如果我们试验目的是用药物处理细胞,比较处理前后某种膜蛋白的表达情况,此时用膜蛋白提取试剂盒提取膜蛋白后再用β-actin做内参似乎就不妥了,因为你根本不知道药物处理前后混杂了多少的胞质蛋白进来。如果没有胞质蛋白混进去的话,β-actin就是检测不到的。
当然做都是可以做,需要带上阴性和阳性的对照组,以区别出是抗体制备的问题还是抗原决定簇被破坏的问题。
原液分装。抗体孵育之前稀释。
做WB的浓度不一定需要很高的。可以设置简单梯度1:200;1:500;1:1000 。看结果再优化
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