The Bovine QPCR Kit is designed for the target-DNA specific detection of bovine matter in food and animal feed ingredients. The assay uses a real-time PCR-based protocol with fluoresce-probe to detect target DNA. It can detect as few as 10 copies of the target DNA in a reaction and it exhibits high specificity for bovine (Fig.1-2, and Table 1). No cross reactivity is observed with other animal species (See Table 2). The detection of target DNA confirms ingredient authenticity or prevents food fraud, ethical issues, or health concerns.
Key Features:
Highly sensitive and specificity for bovine derived DNA.
High efficiency: the optimal systemic conditions for PCR amplifications.
Streamlined protocol: just add DNA template and water.
No cross reactivity with other species
Storage Conditions:
The kit is shipped on ice, and stored at -20°C for long-term storage. Shelf life of 12 months after receipt.
PCR Protocol
1. Set up PCR reaction for each sample in 25 µL
Internal control should be included as below: Positive Control (5 µL /reaction), or Negative Control (5 µL/reaction)
2. Suggested PCR conditions
Fig. 1: Bovine qPCR Amplification
Fig.2: DNA Concentration and qPCR amplification
Table1. Bovine qPCR Sensitivity Data
Table2: Cross-reactivity Survey
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TBS42028: Salmonella qPCR
Additional information
SIZE
100 Reactions
Data Sheet:
TBS42001_Bovine_qPCR kit_V03_2022
MSDS:
TBS42001_CATTLE_QPCR_MSDS 2022
1 review for Tribo™ Bovine QPCR, 100 reactions
5 out of 5
jchong–
Works pretty well, even in low concentrations the kit had clear, strong amplification curves. There were no errors with any parts of the kit.
jchong –
Works pretty well, even in low concentrations the kit had clear, strong amplification curves. There were no errors with any parts of the kit.