Enzymes for Molecular Biology: Enzymes for Molecular Biology
RNase A
Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3"-end of pyrimidine residues
BioSearch Tech (Lucigen/Epicentre)
Catalogue No. | Description | Pack Size | Price | Qty |
|
---|---|---|---|---|---|
MRNA092 | RNase A | 5 µg/µl 2 ml | £63.00 | Quantity | Add to Order |
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RNase A
Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3"-end of pyrimidine residues
BioSearch Tech (Lucigen/Epicentre)
Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3"-end of pyrimidine residues, forming oligoribonucleotides having 3"-terminal pyrimidine-3"-phosphates. Pyrimidine-3"-monophosphates are also released by RNase A cleavage of adjacent pyrimidine nucleotides.
Modified RNA containing pyrimidine-2"-fluoro-dNMPs, such as modified RNA made by in vitro transcription using the DuraScribe® T7 & SP6 Transcription Kits is completely resistant to cleavage by RNase A.
Composition and Concentration
Provided on the basis of weight at 5 mg/ml in Storage Buffer
Storage Buffer
50% glycerol containing 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DDT, 0.1 M NaCl, and 0.1% Triton® X-100
Quality Control
RNase A is free of contaminating DNA exo- and endonuclease activities
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
RNase A
Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3"-end of pyrimidine residues
BioSearch Tech (Lucigen/Epicentre)
protocol
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
RNase A
Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3"-end of pyrimidine residues
BioSearch Tech (Lucigen/Epicentre)
References
- Johnson, M. (1996) EPICENTRE Forum 3(4), 7.
- Shen, V. and Schlessinger, D. (1982) The Enzymes XV (Part B), 501.
- delCardayré, S.B. and Raines, R.T. (1995) Anal. Biochem. 225, 176.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
RNase A
Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3"-end of pyrimidine residues
BioSearch Tech (Lucigen/Epicentre)
Get your Epicentre® Forum - hot off the press!Click here for direct link to Forum listings |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
RNase A
Ribonuclease A (RNase A) is an endoribonuclease that cleaves single-stranded RNA at the 3"-end of pyrimidine residues
BioSearch Tech (Lucigen/Epicentre)
Applications
- Removal of RNA from DNA preparations.1
- RNase protection assays to detect single-basepair mismatches in RNA:RNA and RNA:DNA hybrids.1
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
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