Amount : | Non Profit |
The GATA3 Leeporter™ Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the GATA3 response element, so that the cell line is designed to measure the transcriptional activity of GATA3. As a zinc-finger transcription factor, GATA3 (GATA-binding protein 3) plays a critical role in early and late T cell differentiation, which regulates Th1/Th2 differentiation. GATA3 has been shown to induce Th2 differentiation and repress Th1 differentiation. GATA3 is also known to promote the secretion of IL-4, IL-5 and IL-13 from Th2 cells. The GATA3 induction by phorbol 12-myristate 13-acetate (PMA) is shown in Figure 1.
Content : | Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO. |
Storage condition : | Immediately upon receipt, store in liquid nitrogen. |
Application:
- Monitor the GATA3 signaling pathway activity.
- Screen for activators or inhibitors of the GATA3 signaling pathway.
Culture conditions:
A. Response of GATA3 Leeporter™ – HEK293 cells to phorbol 12-myristate 13-acetate (PMA).
LIMITED USE RESTRICTIONS:
THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.
By use of this product, user agrees to be bound by the terms of this limited use statement.
This product issolely for Internal Research Purposesandnot for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research.The buyer cannot sell, give or otherwise transfer this product to a third party.
Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics (info@abeomics.com).
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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先提取RNA,反转录成cDNA,然后根据目的基因设计PCR引物,通过半定量RT-PCR确定目的基因表达.
正因为检测的是mRNA,所以要先反转录成cDNA才能PCR.
① 在构建载体时,目的基因直接整合到细胞染色体组上,最好不要通过先瞬转在筛选稳定细胞株的这种方法,因为转染效率没有保证
② 高表达载体的构建,哺乳动物表达量一直是它自身的缺点,最好根据高表达载体定向的驯化细胞,提高蛋白表达量
③ 细胞的选择,筛选稳定细胞株我们常用的细胞是CHO,中国仓鼠卵巢细胞,由于CHO具有诸多的优点因此适合用于筛选稳定细胞株,而HEK293细胞则常用于瞬时转染
④ 后期的筛选,双抗预防污染,筛选细胞的时候抗生素浓度一定要做预实验,而且转染的时候不能有抗生素,关于细胞转染 稳定细胞系构建的相关理论
最近因工作需要,欲购进一些生产用的细胞株和瞬转、稳转质粒,大家有什么推荐的品系和公司或网站,谢谢!!!
主要需求:
1.生产或生物制药用的,稳定细胞株如CHO-K1、GS和HEK293/HEK293T,以及与之对应的瞬转、稳转(重组整合)质粒(需详细图谱)。
2.原始或改造细胞株,以及对应质粒,改造细胞株请详述细节,原因,优缺点,安全性等。
3.希望大家积极推荐,实验用、生产用均可,原核、真核都行,网站或品系名称也行,只要大家觉得常用、稳定、安全、高产就行。
4.请有意向的同仁回帖或将详细资料发至我163邮箱:wangqiang23mars@163.com,万分感谢。
然后你得确定,这个细胞株对于你得研究来说不可或缺,具有明确的代表性。
上面两个都没有问题的话,祝你顺利。
如果经费充足的话可以找公司包装病毒,如武汉的普健可以提供各种载体的构建及细胞株构建的技术服务。
暂无品牌问答