GenCatch TM Blood &Tissue Genomic Mini Prep Kit
Catalog# | size | Price | Quantity | |
---|---|---|---|---|
1460050 | 50 Mini Preps | $81.00 | ||
1460250 | 250 Mini Preps | $369.00 |
Description | GenCatch TM Blood &Tissue Genomic Mini-Prep Kitprovides a simple, fast and cost-effectivemethod to purify genomic DNA from varioussamples such as bacteria, yeast, blood, cultured cells, tissue, plasma, serum, buffycoat, lymphocytes, paraffin-embedded tissue and forensic specimens, without phenol/chloroform extraction and ethanol precipitation. Isolated genomic DNA is free of contaminationsand are good substrates for southern blotting, enzymatic and PCR reactions. | ||||
Detailed Components | High quality spin columns, collection tubes together with buffers and protocols. | ||||
Features | Sample Size: Up to 5 ml of bacterial or yeast culture,200 ¦Ìl of whole blood and 30 mg of animal tissue or culture cells can be processedin a single column. Yield: Up to 80 ¦Ìggenomic DNA from bacterial and yeast culture, 120 ¦Ìg from mammalian tissue cultureand 10 ¦Ìg from whole blood sample can be obtained. Quality: GenomicDNA purified is suitable for many downstream molecular manipulations including,genotyping, PCR, enzymatic reactions, southern blotting, etc. Convenient: No phenolextractions or ethanol precipitations required. | ||||
Technical Data | Genomic DNA isolated from livercells with GenCatch TM Blood &Tissue Genomic Mini-PrepKit was run on agarose gel before (second lane) and after (third lane) digestionwith EcoRI.PCR reaction of genomic DNA extractedusing GenCatch TM Blood &Tissue Genomic Mini-Prep Kit from different numbersof HeLa cells (102-106 cells from left to right). The 850 bp fragment is the expected amplification product according to the primer used. |
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哪个正规生物公司DNA提取试剂好用,纯,效果好
这个与琼脂糖电泳有关。提取的DNA分子量很大,可能在40K到100K左右。也有可能在这个范围。但普通的琼脂糖电泳。在大分子的时候根本分别不出来。如果你有DL15000的MARKER你就知道,象那个一万与一万五和条带差别很近。
buffer P1:除去RNA
buffer P2:裂解细胞
buffer P3:沉淀DNA
buffer WA、buffer WB:都是洗涤液(这两个之间有什么区别我也不清楚)
TE:溶解DNA.
大家都用过哪些DNA提取试剂盒,能不能推荐几种
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